The ATIC Antibody (CAB5559) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. RRID AB_2766339 Gene ID 471 Swiss Prot Synonym PURH; AICAR; AICARFT; IMPCHASE; HEL-S-70p; ATIC
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ATIC Antibody
SKU:
CAB5559
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PURH, AICAR, AICARFT, IMPCHASE, HEL-S-70p, ATIC
Positive Sample:
MCF7, SW480, NCI-H460, HepG2, SW620, HT-29, K-562
Cellular Localization:
Cytosol, Extracellular Exosome, Plasma Membrane.
Calculated MW:
65kDa
Observed MW:
62kDa
This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. RRID AB_2766339 Gene ID 471 Swiss Prot Synonym PURH; AICAR; AICARFT; IMPCHASE; HEL-S-70p; ATIC
Purification Method:
Affinity purification
Gene ID:
471
RRID:
AB_2766339
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ATIC Rabbit pAb (CAB5559) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
