Anti-ATOH1 Antibody (CAB6530)

Antibody Name:	Anti-ATOH1 Antibody
Antibody SKU:	CAB6530
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 215-354 of human ATOH1 (NP_005163.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	SW620, HepG2, human pacenta, Mouse liver, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 215-354 of human ATOH1 (NP_005163.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LQTP SGGE QPPP PPAS CKSD HHHL RTAA SYEG GAGN ATAA GAQQ ASGG SQRP TPPG SCRT RFSA PASA GGYS VQLD ALHF STFE DSAL TAMM AQKN LSPS LPGS ILQP VQEE NSKT SPRS HRSD GEFS PHSH YSDS DEAS
Gene ID:	474
Uniprot:	Q92858
Cellular Location:	Nucleus
Calculated MW:	38kDa
Observed MW:	35kDa

Synonyms:	ATOH1, ATH1, HATH1, MATH-1, bHLHa14
Background:	This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47.

UniProt Protein Function:	ATOH1: a conserved transcription factor that regulates secretory cell differentiation in colonic epithelium. Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. May play a role in the differentiation of subsets of neural cells by activating E box- dependent transcription. Efficient DNA binding requires dimerization with another bHLH protein. Loss of ATOH1 expression in the colon of mice initiates colon cancer. ATOH1 has been silenced in most human colon cancers, and reactivation of this gene in cultured human colon cancer cells is anti-proliferative and induces apoptosis.
UniProt Protein Details:	Protein type:Cell development/differentiation; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 4q22 Cellular Component: nucleus Molecular Function:transcription factor activity Biological Process: central nervous system development; positive regulation of neuron differentiation
NCBI Summary:	This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47. [provided by RefSeq, Jul 2008]
UniProt Code:	Q92858
NCBI GenInfo Identifier:	3913115
NCBI Gene ID:	474
NCBI Accession:	Q92858.1
UniProt Secondary Accession:	Q92858,Q14CT9,
UniProt Related Accession:	Q92858
Molecular Weight:	38,160 Da
NCBI Full Name:	Protein atonal homolog 1
NCBI Synonym Full Names:	atonal bHLH transcription factor 1
NCBI Official Symbol:	ATOH1
NCBI Official Synonym Symbols:	ATH1; HATH1; MATH-1; bHLHa14
NCBI Protein Information:	protein atonal homolog 1
UniProt Protein Name:	Protein atonal homolog 1
UniProt Synonym Protein Names:	Class A basic helix-loop-helix protein 14; bHLHa14; Helix-loop-helix protein hATH-1; hATH1
Protein Family:	Protein atonal
UniProt Gene Name:	ATOH1
UniProt Entry Name:	ATOH1_HUMAN

	Western blot analysis of extracts of various cell lines, using ATOH1 Antibody (CAB6530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of MCF7 cells using ATOH1 antibody (CAB6530). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-ATOH1 Antibody (CAB11477)	Human ATOH1 / Protein atonal homolog 1 ELISA Kit
ATOH1 Antibody (PACO07912)	Human Protein atonal homolog 1 (ATOH1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)