The ATP1B1 Monoclonal Antibody (CAB5113) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, carefully developed using rabbit monoclonal technology, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for various research applications.ATP1B1, also known as the sodium/potassium-transporting ATPase subunit beta-1, is a key component of the sodium-potassium pump that maintains cell membrane potential and regulates cellular processes like signaling and transport.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ATP1B1 Monoclonal Antibody
SKU:
CAB5113
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1232
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ATP1B, ATP1B1
Positive Sample:
U-2 OS, Mouse liver, Mouse brain, Rat brain
Cellular Localization:
Cell Membrane.
Calculated MW:
35kDa
Observed MW:
50kDa
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known.
Purification Method
Affinity purification
Gene ID
481
RRID
AB_2863450
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ATP1B1 Rabbit mAb (CAB5113) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from U2OS cells, using ATP1B1 Rabbit mAb (CAB5113) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ATP1B1 Rabbit mAb (CAB5113) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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