Anti-ATP1B2 Antibody (CAB9928)

Antibody Name:	Anti-ATP1B2 Antibody
Antibody SKU:	CAB9928
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 68-290 of human ATP1B2 (NP_001669.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, NCI-H460, SH-SY5Y

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 68-290 of human ATP1B2 (NP_001669.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DHTP KYQD RLAT PGLM IRPK TENL DVIV NVSD TESW DQHV QKLN KFLE PYND SIQA QKND VCRP GRYY EQPD NGVL NYPK RACQ FNRT QLGN CSGI GDST HYGY STGQ PCVF IKMN RVIN FYAG ANQS MNVT CAGK RDED AENL GNFV MFPA NGNI DLMY FPYY GKKF HVNY TQPL VAVK FLNV TPNV EVNV ECRI NAAN IATD DERD KFAG RVAF KLRI NKT
Gene ID:	482
Uniprot:	P14415
Cellular Location:	Cell membrane, Single-pass type II membrane protein
Calculated MW:	33kDa
Observed MW:	42kDa

Synonyms:

ATP1B2, AMOG

Background:

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ATP1B2: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. Belongs to the X(+)/potassium ATPases subunit beta family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: plasma membrane; sodium:potassium-exchanging ATPase complex Molecular Function:ATPase activator activity; ATPase binding; protein binding; sodium:potassium-exchanging ATPase activity Biological Process: cellular potassium ion homeostasis; cellular sodium ion homeostasis; leukocyte migration; positive regulation of ATPase activity; potassium ion import; protein stabilization; transport
NCBI Summary:	The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]
UniProt Code:	P14415
NCBI GenInfo Identifier:	125987795
NCBI Gene ID:	482
NCBI Accession:	P14415.3
UniProt Secondary Accession:	P14415,O60444, A0AV17, A8K278, D3DTQ2,
UniProt Related Accession:	P14415
Molecular Weight:	33,367 Da
NCBI Full Name:	Sodium/potassium-transporting ATPase subunit beta-2
NCBI Synonym Full Names:	ATPase Na+/K+ transporting subunit beta 2
NCBI Official Symbol:	ATP1B2
NCBI Official Synonym Symbols:	AMOG
NCBI Protein Information:	sodium/potassium-transporting ATPase subunit beta-2
UniProt Protein Name:	Sodium/potassium-transporting ATPase subunit beta-2
UniProt Synonym Protein Names:	Adhesion molecule in glia; AMOG; Sodium/potassium-dependent ATPase subunit beta-2
Protein Family:	Sodium/potassium-transporting ATPase
UniProt Gene Name:	ATP1B2
UniProt Entry Name:	AT1B2_HUMAN

	Western blot analysis of extracts of various cell lines, using ATP1B2 antibody (CAB9928) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
	Immunofluorescence analysis of C6 cells using ATP1B2 Rabbit pAb (CAB9928) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-ATP1B2 Antibody (CAB16768)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)