Anti-ATP4B Antibody (CAB10106)

Antibody Name:	Anti-ATP4B Antibody
Antibody SKU:	CAB10106
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 62-291 of human ATP4B (NP_000696.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, SW620, NCI-H460, Mouse small intestine, Mouse liver, Mouse heart, Mouse pancreas, Rat stomach

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 62-291 of human ATP4B (NP_000696.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LMQT VDPY TPDY QDQL RSPG VTLR PDVY GEKG LEIV YNVS DNRT WADL TQTL HAFL AGYS PAAQ EDSI NCTS EQYF FQES FRAP NHTK FSCK FTAD MLQN CSGL ADPN FGFE EGKP CFII KMNR IVKF LPSN GSAP RVDC AFLD QPRE LGQP LQVK YYPP NGTF SLHY FPYY GKKA QPHY SNPL VAAK LLNI PRNA EVAI VCKV MAEH VTFN NPHD PYEG KVEF KLKI EK
Gene ID:	496
Uniprot:	P51164
Cellular Location:	Cell membrane, Single-pass type II membrane protein
Calculated MW:	33kDa
Observed MW:	35kDa

Synonyms:

ATP4B, ATP6B

Background:

The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.

UniProt Protein Function:	ATP4B: belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 13q34 Cellular Component: plasma membrane Molecular Function:hydrogen:potassium-exchanging ATPase activity; protein binding
NCBI Summary:	The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]
UniProt Code:	P51164
NCBI GenInfo Identifier:	1703461
NCBI Gene ID:	496
NCBI Accession:	P51164.1
UniProt Secondary Accession:	P51164,B1B0N8,
UniProt Related Accession:	P51164
Molecular Weight:	33,367 Da
NCBI Full Name:	Potassium-transporting ATPase subunit beta
NCBI Synonym Full Names:	ATPase H+/K+ transporting beta subunit
NCBI Official Symbol:	ATP4B
NCBI Official Synonym Symbols:	ATP6B
NCBI Protein Information:	potassium-transporting ATPase subunit beta
UniProt Protein Name:	Potassium-transporting ATPase subunit beta
UniProt Synonym Protein Names:	Gastric H(+)/K(+) ATPase subunit beta; Proton pump beta chain
Protein Family:	Potassium-transporting ATPase
UniProt Gene Name:	ATP4B
UniProt Entry Name:	ATP4B_HUMAN

	Western blot analysis of extracts of various cell lines, using ATP4B antibody (CAB10106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human stomach using ATP4B antibody (CAB10106) at dilution of 1:100 (40x lens).

Antibodies

ATP4B Antibody (PACO07922)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)