Anti-BAG1 Antibody (CAB1104)

Antibody Name:	Anti-BAG1 Antibody
Antibody SKU:	CAB1104
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human BAG1 (NP_001165886.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	SW620, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human BAG1 (NP_001165886.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNRS QEVT RDEE STRS EEVT REEM AAAG LTVT VTHS NEKH DLHV TSQQ GSSE PVVQ DLAQ VVEE VIGV PQSF QKLI FKGK SLKE METP LSAL GIQD GCRV MLIG KKNS PQEE VELK KLKH LEKS VEKI ADQL EELN KELT GIQQ GFLP KDLQ AEAL CKLD RRVK ATIE QFMK ILEE IDTL ILPE NFKD SRLK RKGL VKKV QAFL AECD TVEQ NICQ ETER LQST NFAL AE
Gene ID:	573
Uniprot:	Q99933
Cellular Location:	Cytoplasm, Cytoplasm, Nucleus
Calculated MW:	25kDa/31kDa/34kDa/38kDa
Observed MW:	43kDa

Synonyms:

BAG1, BAG-1, HAP, RAP46

Background:

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X.

UniProt Protein Function:	BAG1: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. Homodimer. Forms a heteromeric complex with HSP70/HSC70. Binds to the ATPase domain of HSP/HSC70 chaperones. Isoform 1, isoform 3 and isoform 4 but not isoform 2 interact with HSPA8/HSC70. Interacts with NR3C1. Interacts with the N-terminal region of STK19. Interacts with PPP1R15A. Interacts with BCL2 in an ATP-dependent manner. Isoform 2 does not interact with BCL2. Up-regulated during differentiation of bladder epithelial cells and down-regulated during differentiation of prostate epithelium. Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 9p12 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding; chaperone binding; receptor signaling protein activity Biological Process: cell surface receptor linked signal transduction; apoptosis; negative regulation of apoptosis
NCBI Summary:	The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X. [provided by RefSeq, Feb 2010]
UniProt Code:	Q99933
NCBI GenInfo Identifier:	296439462
NCBI Gene ID:	573
NCBI Accession:	Q99933.4
UniProt Secondary Accession:	Q99933,O75315, Q14414, Q53H32, Q5VZE8, Q5VZE9, Q5VZF0 Q96TG2, Q9Y2V4,
UniProt Related Accession:	Q99933
Molecular Weight:	345
NCBI Full Name:	BAG family molecular chaperone regulator 1
NCBI Synonym Full Names:	BCL2-associated athanogene
NCBI Official Symbol:	BAG1
NCBI Official Synonym Symbols:	HAP; BAG-1; RAP46
NCBI Protein Information:	BAG family molecular chaperone regulator 1; Bcl-2-binding protein; receptor-associated protein, 46-KD; Bcl-2 associating athanogene-1 protein; glucocortoid receptor-associated protein RAP46
UniProt Protein Name:	BAG family molecular chaperone regulator 1
UniProt Synonym Protein Names:	Bcl-2-associated athanogene 1
Protein Family:	BAG family molecular chaperone regulator
UniProt Gene Name:	BAG1
UniProt Entry Name:	BAG1_HUMAN

	Western blot analysis of extracts of various cell lines, using BAG1 antibody (CAB1104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human kidney cancer using BAG1 Antibody (CAB1104) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney damage using BAG1 Antibody (CAB1104) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using BAG1 Antibody (CAB1104) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using BAG1 antibody (CAB1104). Blue: DAPI for nuclear staining.

Antibodies

BAG1 Antibody (PACO02048)

BAG1 Antibody (PACO07974)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)