Anti-BAG4 Antibody (CAB17052)

Antibody Name:	Anti-BAG4 Antibody
Antibody SKU:	CAB17052
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BAG4 (NP_001191807.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BAG4 (NP_001191807.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSAL RRSG YGPS DGPS YGRY YGPG GGDV PVHP PPPL YPLR PEPP QPPI SWRV RGGG PAET TWLG EGGG GDGY YPSG GAWP EPGR AGGS HQSL NSYT NGAY
Gene ID:	9530
Uniprot:	O95429
Cellular Location:
Calculated MW:	45kDa/49kDa
Observed MW:	Refer to figures

Synonyms:

BAG4, BAG-4, SODD

Background:

The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

UniProt Protein Function:	BAG4: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Prevents constitutive TNFRSF1A signaling. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Apoptosis Chromosomal Location of Human Ortholog: 8p11.23 Cellular Component: cytosol; nucleus; plasma membrane Molecular Function:adenyl-nucleotide exchange factor activity; protein binding; receptor signaling protein activity; ubiquitin protein ligase binding Biological Process: negative regulation of apoptosis; protein folding; tumor necrosis factor-mediated signaling pathway
NCBI Summary:	The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]
UniProt Code:	O95429
NCBI GenInfo Identifier:	12643661
NCBI Gene ID:	9530
NCBI Accession:	O95429.1
UniProt Secondary Accession:	O95429,O95818, B4E217,
UniProt Related Accession:	O95429
Molecular Weight:	45,410 Da
NCBI Full Name:	BAG family molecular chaperone regulator 4
NCBI Synonym Full Names:	BCL2 associated athanogene 4
NCBI Official Symbol:	BAG4
NCBI Official Synonym Symbols:	SODD; BAG-4
NCBI Protein Information:	BAG family molecular chaperone regulator 4
UniProt Protein Name:	BAG family molecular chaperone regulator 4
UniProt Synonym Protein Names:	Bcl-2-associated athanogene 4; Silencer of death domains
Protein Family:	BAG family molecular chaperone regulator
UniProt Gene Name:	BAG4

Western blot analysis of extracts of various cell lines, using BAG4 antibody (CAB17052) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

Anti-BAG4 Antibody (CAB17053)

BAG4 Antibody (PACO06275)

BAG4 Antibody (PACO07976)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)