Description
BCG Albumin Assay Kit (BA0029) (BA0029)
The BCG Albumin Assay Kit (SKU: BA0029) is designed to measure albumin directly in biological samples without any pretreatment. Albumin is the most abundant plasma protein, accounting for around 60% of total serum protein, and plays key physiological roles including maintenance of colloid osmotic pressure and the binding and transport of fatty acids, bile acids, bilirubin, calcium and drugs. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephrotic syndrome and malnutrition. This kit uses bromocresol green, which forms a coloured complex specifically with albumin; the intensity of the colour measured at 620 nm is directly proportional to the albumin concentration. The optimised formulation substantially reduces interference from substances in raw samples, and the single working-reagent, five-minute procedure is readily automated for high-throughput work. It can be run in either 96-well plate or cuvette format.
| Product Name: | BCG Albumin Assay Kit (BA0029) |
| SKU: | BA0029 |
| Detection Method: | Colorimetric; bromocresol green complex measured at 620 nm |
| Detection Range: | 0.01 g/dL (1.5 µM) to 5 g/dL (750 µM) albumin |
| Sample Type: | Serum, plasma, urine, biological preparations |
| Species Reactivity: | All |
| Assay Time: | 5 min incubation |
| Kit Size: | 250 Assays |
| Equipment Required: | Microplate reader |
| Storage: | Store Reagent at 4°C and Standard at -20°C. |
| Shelf Life: | 12 months after receipt. |
| Shipping: | Room Temperature |
A colorimetric assay for the quantitative determination of albumin directly in biological samples. The assay uses bromocresol green, which forms a coloured complex specifically with albumin; the intensity measured at 620 nm is directly proportional to albumin concentration. Detection range is 0.01 g/dL (1.5 µM) to 5 g/dL (750 µM).
- Sensitive and accurate: uses as little as 5 µL of sample; detection range 0.01 to 5 g/dL albumin.
- Simple and high-throughput: a single working reagent and a 5-minute incubation; readily automated in 96-well plates.
- Improved reagent stability and versatility: enhanced reagent and signal stability; cuvette or 96-well plate assay.
- No interference in biological samples: no pretreatment required, even in the presence of lipid and protein.
- Albumin in serum, plasma, urine and biological preparations.
- Drug discovery and pharmacology: effects of drugs on albumin metabolism.
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Bring the reagent to room temperature and shake before use. |
| 2 | Dilute the standards in distilled water as shown in the standards table and dilute serum and plasma samples 2-fold. Transfer 5 µL of diluted standards and samples to wells of a clear-bottom plate. |
| 3 | Add 200 µL working reagent and tap lightly to mix, avoiding bubbles. |
| 4 | Incubate 5 min at room temperature and read the optical density at 570-670 nm (peak absorbance at 620 nm). |
| 5 | Cuvette format: transfer 20 µL Blank, Standards and samples to tubes, add 1000 µL working reagent, incubate 5 min and read at 620 nm. |
Subtract the blank OD (water, standard #8) from the standard OD values and plot OD against concentration. Use the standard curve to determine the sample albumin concentration. Conversions: 0.1 g/dL albumin equals 15 µM, 0.1% or 1000 ppm.
| Component | Quantity | Storage |
| Reagent | 50 mL | 4°C |
| Albumin Standard (5 g/dL BSA) | 1 mL | -20°C |
