The Canine Albumin (ALB) ELISA Kit from Assay Genie is a cutting-edge tool designed for precise measurement of albumin levels in canine serum, plasma, and other biological samples. With its advanced technology and robust performance, this ELISA kit ensures accurate and reliable results, making it an indispensable resource for a wide array of research studies. Albumin is a vital protein that serves various functions in the body, including maintaining colloidal osmotic pressure, carrying essential molecules, and regulating pH balance. Fluctuations in albumin levels can indicate underlying health conditions such as liver disease, kidney disorders, and nutritional deficiencies, highlighting the importance of monitoring albumin levels in veterinary diagnostics and research endeavors.
This Canine Albumin ELISA Kit offers researchers a comprehensive solution for evaluating albumin concentrations in canine samples with precision and efficiency. By facilitating in-depth investigations into canine health and disease mechanisms, this kit supports advancements in veterinary medicine and enhances our understanding of albumin's role in canine physiology.
Product Name:
Canine ALB (Albumin) ELISA Kit
SKU:
AEFI00054
Reactivity:
Canine
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.234 ng/mL
Range:
0.39-25 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-103%
85-103%
94-99%
EDTA Plasma (n = 5)
82-101%
84-98%
87-98%
Heparin Plasma (n = 5)
86-100%
81-99%
82-96%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
89-105
98
EDTA Plasma (n = 5)
86-100
94
Heparin Plasma (n = 5)
91-101
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
