The Canine Albumin (ALB) ELISA Kit from AssayGenie is a state-of-the-art product designed for the accurate quantification of albumin levels in canine serum, plasma, and tissue culture samples. This kit offers high sensitivity and specificity, allowing for precise and reproducible results, making it an invaluable tool for a variety of research applications.Albumin is a vital protein found in the blood that plays a crucial role in maintaining oncotic pressure, transporting hormones and drugs, and regulating pH levels.
Abnormalities in albumin levels are associated with various health conditions, including liver disease, kidney dysfunction, and malnutrition. By accurately measuring albumin levels in canine samples, researchers can gain valuable insights into the health and well-being of these animals, as well as monitor the progression of diseases and treatment outcomes. The Canine Albumin (ALB) ELISA Kit is a valuable resource for veterinary research and diagnostic laboratories seeking to improve animal health and welfare.
Product Name:
Canine Albumin (ALB) ELISA Kit
Product Code:
CNDL00005
Size:
96 Assays
Target:
ALB
Synonyms:
Serum albumin
Detection Method:
Competition
Range:
1.56-100ug/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
