Anti-Bcl-W Antibody (CAB13471)

Antibody Name:	Anti-Bcl-W Antibody
Antibody SKU:	CAB13471
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human Bcl-W (NP_001186768.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse intestine

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human Bcl-W (NP_001186768.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATP ASAP DTRA LVAD FVGY KLRQ KGYV CGAG PGEG PAAD PLHQ AMRA AGDE FETR FRRT FSDL AAQL HVTP GSAQ QRFT QVSD ELFQ GGPN WGRL VAFF VFGA ALCA ESVN KEME PLVG QVQE WMVA YLET RLAD WIHS SGGW AEFT ALYG DGAL EEAR RLRE GNWA SVRT VLTG AVAL GALV TVGA FFAS K
Gene ID:	599
Uniprot:	Q92843
Cellular Location:	Mitochondrion membrane, Peripheral membrane protein
Calculated MW:	20kDa/37kDa
Observed MW:	27kDa

Synonyms:

BCL2L2, BCL-W, BCL2-L-2, BCLW, PPP1R51

Background:

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene.

UniProt Protein Function:	Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.
NCBI Summary:	This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene. [provided by RefSeq, Dec 2010]
UniProt Code:	Q92843
NCBI GenInfo Identifier:	296434404
NCBI Gene ID:	599
NCBI Accession:	Q92843.2
UniProt Secondary Accession:	Q92843,Q2M3U0, Q5U0H4, A8K0F4,
UniProt Related Accession:	Q92843
Molecular Weight:	37,171 Da
NCBI Full Name:	Bcl-2-like protein 2
NCBI Synonym Full Names:	BCL2 like 2
NCBI Official Symbol:	BCL2L2
NCBI Official Synonym Symbols:	BCLW; BCL-W; PPP1R51; BCL2-L-2
NCBI Protein Information:	bcl-2-like protein 2
UniProt Protein Name:	Bcl-2-like protein 2
UniProt Synonym Protein Names:	Apoptosis regulator Bcl-W
Protein Family:	Bcl-2-like protein
UniProt Gene Name:	BCL2L2
UniProt Entry Name:	B2CL2_HUMAN

	Western blot analysis of extracts of mouse intestine, using Bcl-W antibody (CAB13471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat liver using Bcl-W antibody (CAB13471) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human mammary cancer using Bcl-W antibody (CAB13471) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using Bcl-W antibody (CAB13471) at dilution of 1:100 (40x lens).

Antibodies

Anti-Bcl-W Antibody (CAB1158)

BCL2L2 Antibody (PACO00460)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)