Western blot analysis of Mouse brain, using Bcl9 Rabbit pAb (CAB22868) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Bcl9 Polyclonal Antibody (CAB22868) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and is highly specific to human samples, making it an ideal choice for Western blot applications. By targeting the BCL9 protein, this antibody allows for the detection and analysis of Wnt signaling pathways in various cell types.The Wnt signaling pathway plays a critical role in cell proliferation, differentiation, and migration, making it a key player in development, tissue regeneration, and disease progression. Dysregulation of this pathway is often associated with various cancers and other diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Bcl9 Polyclonal Antibody
SKU:
CAB22868
Size:
20μL, 100μL
Reactivity:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LGS, Bcl9
Positive Sample:
Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
149kDa
Observed MW:
149kDa
BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.
Purification Method
Affinity purification
Gene ID
607
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse brain, using Bcl9 Rabbit pAb (CAB22868) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
