The Bcl9 Polyclonal Antibody (CAB22868) is a high-quality antibody developed for reliable detection and analysis of target proteins. BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Bcl9 Polyclonal Antibody
SKU:
CAB22868
Size:
100μL, 20μL
Reactivity:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LGS, Bcl9
Positive Sample:
Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
149kDa
Observed MW:
149kDa
BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.
Purification Method
Affinity purification
Gene ID
607
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse brain, using Bcl9 Rabbit pAb (CAB22868) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
