Beta-4 Spectrin Antibody [N393/2 (Formerly sold as S393-2)]
Beta-4 Spectrin Antibody [N393/2 (Formerly sold as S393-2)] is a mouse monoclonal antibody directed against Beta-4 Spectrin, offered by Assay Genie for research applications. Reported applications include WB, IHC and ICC/IF. Reported reactivity: Human, Mouse and Rat. Supplied as IgG1, purified by Protein G.
Beta IV spectrin (SPTBN4) is a non-erythrocytic member of the spectrin family, a group of cytoskeletal proteins essential for maintaining cell shape, membrane integrity, and the spatial organization of intracellular components. While spectrins are best known for their role in red blood cells, beta IV spectrin is predominantly expressed in the nervous system, particularly in the cerebellum, spinal cord, and brain, as well as in pancreatic islets. In neurons, beta IV spectrin localizes to the nuclear matrix, cytoplasmic vesicles, and promyelocytic leukemia (PML) nuclear bodies. It plays a critical role in stabilizing membrane domains and organizing ion channels at the nodes of Ranvier—specialized axonal segments essential for saltatory conduction. This structural role is vital for maintaining the fidelity of neuronal signaling. Disruption of beta IV spectrin function has been linked to neurodevelopmental and neurodegenerative disorders. Mutations in the SPTBN4 gene are associated with severe neurological phenotypes, including motor dysfunction, intellectual disability, and epilepsy. Given its role in axonal architecture and signal propagation, beta IV spectrin is emerging as a key player in the pathophysiology of diseases such as multiple sclerosis, peripheral neuropathies, and potentially ALS. As a cytoskeletal scaffold critical for neural connectivity and excitability, beta IV spectrin represents a promising target for research into the mechanisms underlying neurodegeneration and for the development of neuroprotective strategies. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
Product Name:
Beta-4 Spectrin Antibody [N393/2 (Formerly sold as S393-2)]
Product SKU:
STMA00165
Size:
100 µg
Target:
Beta-4 Spectrin
Host Species:
Mouse
Antibody Type:
Monoclonal
Clone ID:
N393/2 (Formerly sold as S393-2)
Research Areas:
Neuroscience, Cell Signaling, Scaffold Proteins
Buffer:
PBS pH7.4, 50% glycerol, 0.1% sodium azide
Storage & Shipping:
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:
Liquid
Applications:
WB, IHC, ICC/IF
Antibody Isotype:
IgG1
Purification:
Protein G Purified
Concentration:
1 mg/ml
Reactivity:
Human, Mouse, Rat
Recommended Dilution:
WB (1:1000); optimal dilutions for assays should be determined by the user.
Specificity:
Detects ~>200kDa. Does not cross react with other Beta-spectrins
Guarantee:
12 months from date of dispatch
Immunogen:
Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin
1. Genes and mapped phenotypes. (n.d.). Retrieved March 12, 2015, from http://www.ncbi.nlm.nih.gov/gene/57731 2. Yang Y., Lacas-Gervais S., Morest D.K., Solimena M., and Rasband M.N. (2004) J Neurosci. 24(33): 7230-7240.
