Biotinylated Recombinant Human Latent TGF-beta 1 Protein
The Biotinylated Recombinant Human Latent TGF-beta 1 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Biotinylated Latent TGF-beta 1 function and its implications in health and disease.
This product (SKU: RPCB0445) is produced using advanced expression systems and features a N-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 44.2 kDa with an observed molecular weight of 40-50 kDa (LAP), 13-15 kDa (Mature TGF beta 1) under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant Human Latent TGF-beta 1 Protein
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human Latent TGF-beta 1 Protein (RPCB0445), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin?like convertase processes the proprotein to generate an N?terminal 249 aa latency?associated peptide (LAP) and a C?terminal 112 aa mature TGF? beta 1. Disulfide?linked homodimers of LAP and TGF? beta 1 remain non?covalently associated after secretion, forming the small latent TGF? beta 1 complex.
Biotinylated Recombinant Human Latent TGF-beta 1 Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Biotinylated Human Latent TGF beta 1 is greater than 93% as determined by SEC-HPLC.
Immobilized Human ITGAV&ITGB8, His Tag at 5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.39ug/mL determined by ELISA.
