Anti-BMPR1B Antibody (CAB2005)

Antibody Name:	Anti-BMPR1B Antibody
Antibody SKU:	CAB2005
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human BMPR1B (NP_001194.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, SH-SY5Y

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human BMPR1B (NP_001194.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLLR SAGK LNVG TKKE DGES TAPT PRPK VLRC KCHH HCPE DSVN NICS TDGY CFTM IEED DSGL PVVT SGCL GLEG SDFQ CRDT PIPH QRRS IECC TERN ECNK DLHP TLPP LKNR DFVD GPIH HR
Gene ID:	658
Uniprot:	O00238
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	56kDa/60kDa
Observed MW:	51KDa

Synonyms:

ALK-6, ALK6, AMDD, BDA1D, BDA2, CDw293, BMPR1B

Background:

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. Several transcript variants encoding two different isoforms have been found for this gene.

UniProt Protein Function:	BMPR1B: a kinase of the STKR family. A type I cell-surface receptor for bone morphogenetic protein. Expressed at ighest level in the prostate. May be upregulated by androgen stimulation in cells which expresses the androgen receptor.
UniProt Protein Details:	Protein type:Protein kinase, TKL; EC 2.7.11.30; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Kinase, protein; TKL group; STKR family; Type1 subfamily Chromosomal Location of Human Ortholog: 4q22-q24 Cellular Component: integral to plasma membrane; plasma membrane; receptor complex Molecular Function:protein serine/threonine kinase activity; protein binding; transforming growth factor beta receptor activity, type I; metal ion binding; SMAD binding; transmembrane receptor protein serine/threonine kinase activity; ATP binding; receptor signaling protein serine/threonine kinase activity Biological Process: chondrocyte development; positive regulation of bone mineralization; limb morphogenesis; protein amino acid phosphorylation; BMP signaling pathway; positive regulation of osteoblast differentiation; ovulation cycle; eye development; dorsal/ventral pattern formation; retina development in camera-type eye; proteoglycan biosynthetic process; ovarian cumulus expansion; positive regulation of cell differentiation; inflammatory response; skeletal development; retinal ganglion cell axon guidance; cartilage condensation Disease: Chondrodysplasia, Acromesomelic, With Genital Anomalies; Brachydactyly, Type A2
NCBI Summary:	This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
UniProt Code:	O00238
NCBI GenInfo Identifier:	6226778
NCBI Gene ID:	658
NCBI Accession:	O00238.1
UniProt Secondary Accession:	O00238,P78366, B2R953, B4DSV1,
UniProt Related Accession:	O00238
Molecular Weight:	502
NCBI Full Name:	Bone morphogenetic protein receptor type-1B
NCBI Synonym Full Names:	bone morphogenetic protein receptor, type IB
NCBI Official Symbol:	BMPR1B
NCBI Official Synonym Symbols:	ALK6; ALK-6; CDw293
NCBI Protein Information:	bone morphogenetic protein receptor type-1B; BMPR-1B; BMP type-1B receptor; activin receptor-like kinase 6; serine/threonine receptor kinase
UniProt Protein Name:	Bone morphogenetic protein receptor type-1B
UniProt Synonym Protein Names:	CD_antigen: CDw293
Protein Family:	Bone morphogenetic protein receptor
UniProt Gene Name:	BMPR1B
UniProt Entry Name:	BMR1B_HUMAN

	Western blot analysis of extracts of various cell lines, using BMPR1B Rabbit pAb (CAB2005) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human gastric cancer using BMPR1B antibody (CAB2005) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded rat ovary using BMPR1B antibody (CAB2005) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using BMPR1B antibody (CAB2005) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of L929 cells using BMPR1B antibody (CAB2005) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-BMPR1B Antibody (CAB17858)

Anti-BMPR1B Antibody (CAB17295)

BMPR1B Antibody (PACO07415)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)