The Bovine E2 (Estradiol) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of estradiol levels in bovine serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it ideal for a variety of research applications in the field of veterinary medicine and reproductive biology. Estradiol is a key hormone in the estrous cycle of cattle, playing a critical role in ovarian follicle development, ovulation, and maintenance of pregnancy.
Monitoring estradiol levels is essential for assessing reproductive health, optimizing breeding programs, and diagnosing hormonal disorders in cattle. Whether studying the impact of environmental factors on hormone levels or investigating reproductive diseases in livestock, the Bovine E2 (Estradiol) ELISA Kit offers a valuable tool for researchers looking to advance their understanding of bovine reproductive physiology and develop targeted interventions for improving animal health and productivity.
Product Name:
Bovine E2 (Estradiol) ELISA Kit
SKU:
BOFI00055
Reactivity:
Bovine
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
7.5 pg/mL
Range:
12.5-800 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 10)
87-104%
86-100%
81-94%
EDTA Plasma (n = 10)
87-103%
84-100%
88-98%
Heparin Plasma (n = 10)
87-105%
86-98%
84-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 10)
86-101
93
EDTA Plasma (n = 10)
92-105
98
Heparin Plasma (n = 10)
88-99
93
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Lyophilized)
1 vial
1 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
