The Bovine Ribonuclease A (RNase1) ELISA Kit is specially designed for the precise detection of bovine ribonuclease A levels in bovine serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results, making it perfect for a variety of research applications.Bovine ribonuclease A, also known as RNase1, is an important enzyme involved in RNA degradation and plays a crucial role in various biological processes. It is commonly used in research studies related to RNA biology, gene expression, and protein purification.
The accurate measurement of bovine ribonuclease A levels is essential for understanding its function and its potential implications in different biological pathways.Whether studying RNA processing, gene regulation, or protein degradation, the Bovine Ribonuclease A (RNase1) ELISA Kit provides researchers with a reliable tool for investigating the role of bovine ribonuclease A in various biological processes.
Product Name:
Bovine Ribonuclease A (RNASE1) ELISA Kit
Product Code:
BODL00151
Size:
96 Assays
Target:
RNASE1
Synonyms:
RNASE, Rnase-A, RNase UpI-1, RIB-1, Ribonuclease,RNase A Family,1(Pancreatic)
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
