Anti-BTAF1 Antibody (CAB5811)

Antibody Name:	Anti-BTAF1 Antibody
Antibody SKU:	CAB5811
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP ChIP
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1600-1849 of human BTAF1 (NP_003963.1).

Application:	WB IHC IP ChIP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:20 - 1:50 ChIP 1:20 - 1:100
Reactivity:	Human, Rat
Positive Samples:	HepG2, HeLa, MCF7, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1600-1849 of human BTAF1 (NP_003963.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LAVQ NSSL HDIQ HAPK LSAL KQLL LDCG LGNG STSE SGTE SVVA QHRI LIFC QLKS MLDI VEHD LLKP HLPS VTYL RLDG SIPP GQRH SIVS RFNN DPSI DVLL LTTH VGGL GLNL TGAD TVVF VEHD WNPM RDLQ AMDR AHRI GQKR VVNV YRLI TRGT LEEK IMGL QKFK MNIA NTVI SQEN SSLQ SMGT DQLL DLFT LDKD GKAE KADT STSG KASM KSIL ENLS DLWD QEQY DSEY SLEN FMHS LK
Gene ID:	9044
Uniprot:	O14981
Cellular Location:	Nucleus
Calculated MW:	76kDa/206kDa
Observed MW:	250kDa

Synonyms:	BTAF1, MOT1, TAF(II)170, TAF172, TAFII170
Background:	This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters.

UniProt Protein Function:	TAF172: Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner. Associates with TBP to form B-TFIID. Binds DRAP1. Belongs to the SNF2/RAD54 helicase family.
UniProt Protein Details:	Protein type:Transcription initiation complex; Helicase; EC 3.6.4.-; EC 3.6.1.- Chromosomal Location of Human Ortholog: 10q22-q23 Cellular Component: nucleus Molecular Function:DNA binding; transcription factor activity; helicase activity; ATP binding Biological Process: metabolic process; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters. [provided by RefSeq, Sep 2011]
UniProt Code:	O14981
NCBI GenInfo Identifier:	12643543
NCBI Gene ID:	9044
NCBI Accession:	O14981.2
UniProt Secondary Accession:	O14981,O43578, B4E0W6,
UniProt Related Accession:	O14981
Molecular Weight:	1849
NCBI Full Name:	TATA-binding protein-associated factor 172
NCBI Synonym Full Names:	BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa
NCBI Official Symbol:	BTAF1
NCBI Official Synonym Symbols:	MOT1; TAF172; TAFII170; TAF(II)170
NCBI Protein Information:	TATA-binding protein-associated factor 172; Mot1 homolog; TBP-associated factor 172; ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit; BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae)
UniProt Protein Name:	TATA-binding protein-associated factor 172
UniProt Synonym Protein Names:	ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit; TAF(II)170; TBP-associated factor 172; TAF-172
Protein Family:	TATA-binding protein-associated factor
UniProt Gene Name:	BTAF1
UniProt Entry Name:	BTAF1_HUMAN

	Western blot analysis of extracts of various cell lines, using BTAF1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
	Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug BTAF1 antibody at a dilition of 1:500.
	Chromatin immunoprecipitation analysis of extracts of HeLa cells, using BTAF1 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)