Anti-C1GALT1C1 Antibody (CAB7590)

Antibody Name:	Anti-C1GALT1C1 Antibody
Antibody SKU:	CAB7590
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-190 of human C1GALT1C1 (NP_689905.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-190 of human C1GALT1C1 (NP_689905.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IGHG NRMH HHEH HHLQ APNK EDIL KISE DERM ELSK SFRV YCII LVKP KDVS LWAA VKET WTKH CDKA EFFS SENV KVFE SINM DTND MWLM MRKA YKYA FDKY RDQY NWFF LARP TTFA IIEN LKYF LLKK DPSQ PFYL GHTI KSGD LEYV GMEG GIVL S
Gene ID:	29071
Uniprot:	Q96EU7
Cellular Location:	Membrane, Single-pass type II membrane protein
Calculated MW:	36kDa
Observed MW:	30kDa

Synonyms:

C1GALT1C1, C1GALT2, C38H2-L1, COSMC, HSPC067, MST143, TNPS

Background:

This gene encodes a type II transmembrane protein that is similar to the core 1 beta1, 3-galactosyltransferase 1, which catalyzes the synthesis of the core-1 structure, also known as Thomsen-Friedenreich antigen, on O-linked glycans. This gene product lacks the galactosyltransferase activity itself, but instead acts as a molecular chaperone required for the folding, stability and full activity of the core 1 beta1, 3-galactosyltransferase 1. Mutations in this gene have been associated with Tn syndrome. Alternatively spliced transcript variants encoding the same protein have been identified.

UniProt Protein Function:	C1GALT1C1: Probable chaperone required for the generation of 1 O- glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Probably acts as a specific molecular chaperone assisting the folding/stability of core 1 beta-3-galactosyltransferase (C1GALT1). Defects in C1GALT1C1 are the cause of Tn syndrome (TNSYN). Tn syndrome is a rare autoimmune disease caused by somatic mutation in the C1GALT1C1 gene in which subpopulations of blood cells of all lineages carry an incompletely glycosylated membrane glycoprotein, i.e. the Tn antigen. Since leukocytes and platelets are affected as well as red cells, anemia, leukopenia and thrombocytopenia are features. Tn-polyagglutinability is sometimes associated with leukemia or is a preleukemic state. Belongs to the glycosyltransferase 31 family. Beta3- Gal-T subfamily.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: Xq24 Cellular Component: Golgi membrane; integral to membrane Molecular Function:protein binding; polypeptide N-acetylgalactosaminyltransferase activity Biological Process: platelet activation; protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; positive regulation of transferase activity; post-translational protein modification Disease: Tn Polyagglutination Syndrome
NCBI Summary:	This gene encodes a type II transmembrane protein that is similar to the core 1 beta1,3-galactosyltransferase 1, which catalyzes the synthesis of the core-1 structure, also known as Thomsen-Friedenreich antigen, on O-linked glycans. This gene product lacks the galactosyltransferase activity itself, but instead acts as a molecular chaperone required for the folding, stability and full activity of the core 1 beta1,3-galactosyltransferase 1. Mutations in this gene have been associated with Tn syndrome. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Dec 2009]
UniProt Code:	Q96EU7
NCBI GenInfo Identifier:	74751849
NCBI Gene ID:	29071
NCBI Accession:	Q96EU7.1
UniProt Secondary Accession:	Q96EU7,Q8WWS3, Q9NZX1, A8K246,
UniProt Related Accession:	Q96EU7
Molecular Weight:	36,382 Da
NCBI Full Name:	C1GALT1-specific chaperone 1
NCBI Synonym Full Names:	C1GALT1-specific chaperone 1
NCBI Official Symbol:	C1GALT1C1
NCBI Official Synonym Symbols:	TNPS; COSMC; MST143; C1GALT2; HSPC067; C38H2-L1
NCBI Protein Information:	C1GALT1-specific chaperone 1; C1Gal-T2; C38H2-like protein 1; beta 1,3-galactosyltransferase 2; core 1 beta1,3-galactosyltransferase 2; core 1 beta3-Gal-T2; core 1 beta3-galactosyltransferase-specific molecular chaperone
UniProt Protein Name:	C1GALT1-specific chaperone 1
UniProt Synonym Protein Names:	C38H2-like protein 1; C38H2-L1; Core 1 beta1,3-galactosyltransferase 2; C1Gal-T2; C1GalT2; Core 1 beta3-Gal-T2; Core 1 beta3-galactosyltransferase-specific molecular chaperone
Protein Family:	C1GALT1-specific chaperone
UniProt Gene Name:	C1GALT1C1
UniProt Entry Name:	C1GLC_HUMAN

	Western blot analysis of extracts of various cell lines, using C1GALT1C1 antibody (CAB7590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using C1GALT1C1 antibody (CAB7590) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using C1GALT1C1 antibody (CAB7590) at dilution of 1:100 (40x lens).

Antibodies

C1GALT1C1 Antibody (PACO08089)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)