The C9orf114 Antibody (CAB18460) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables miRNA binding activity. Involved in maintenance of centrosome location and production of miRNAs involved in gene silencing by miRNA. Located in kinetochore; mitotic spindle; and spindle pole centrosome.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
C9orf114 Antibody
SKU:
CAB18460
Size:
100μL, 20μL
Reactivity:
Human, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CENP32, CENP-32, HSPC109, C9orf114
Positive Sample:
U-87MG, K-562
Cellular Localization:
Cytoplasm, Mitotic Spindle, Spindle Pole Centrosome.
Calculated MW:
42kDa
Observed MW:
42kDa
Enables miRNA binding activity. Involved in maintenance of centrosome location and production of miRNAs involved in gene silencing by miRNA. Located in kinetochore; mitotic spindle; and spindle pole centrosome.
Purification Method
Affinity purification
Gene ID
51490
RRID
AB_2862227
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using C9orf114 Rabbit pAb (CAB18460) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using C9orf114 Rabbit pAb (CAB18460) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
