Anti-CGRP Antibody (CAB5542)

Antibody Name:	Anti-CGRP Antibody
Antibody SKU:	CAB5542
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-141 of human CGRP (NP_001029124.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	PC-12

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-141 of human CGRP (NP_001029124.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGFQ KFSP FLAL SILV LLQA GSLH AAPF RSAL ESSP ADPA TLSE DEAR LLLA ALVQ DYVQ MKAS ELEQ EQER EGSS LDSP RSKR CGNL STCM LGTY TQDF NKFH TFPQ TAIG VGAP GKKR DMSS DLER DHRP HVSM PQNA N
Gene ID:	796
Uniprot:	P01258/P06881
Cellular Location:	Secreted
Calculated MW:	13kDa/15kDa
Observed MW:	16kDa

Synonyms:

CALCA, CALC1, CGRP, CGRP-I, CGRP1, CT, KC, PCT

Background:

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CALCA: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 11p15.2 Cellular Component: extracellular region; extracellular space Molecular Function:calcitonin receptor binding; identical protein binding; protein binding Biological Process: activation of protein kinase activity; cellular protein metabolic process; elevation of cytosolic calcium ion concentration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); embryo implantation; monocyte chemotaxis; negative regulation of bone resorption; negative regulation of transcription, DNA-dependent; positive regulation of adenylate cyclase activity
NCBI Summary:	This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]
UniProt Code:	P01258
NCBI GenInfo Identifier:	322510018
NCBI Gene ID:	796
NCBI Accession:	P01258.2
UniProt Secondary Accession:	P01258,Q13935, Q13937, Q52LX7, B7ZL39,
UniProt Related Accession:	P01258,P06881
Molecular Weight:	38.6kDa
NCBI Full Name:	Calcitonin
NCBI Synonym Full Names:	calcitonin related polypeptide alpha
NCBI Official Symbol:	CALCA
NCBI Official Synonym Symbols:	CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I
NCBI Protein Information:	calcitonin; calcitonin gene-related peptide 1
UniProt Protein Name:	Calcitonin
UniProt Synonym Protein Names:	CalcitoninKatacalcinAlternative name(s):Calcitonin carboxyl-terminal peptide; CCP
UniProt Gene Name:	CALCA

	Western blot analysis of extracts of Jurkat cells, using CGRP antibody (CAB5542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of C6 cells using CGRP antibody (CAB5542) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using CGRP antibody (CAB5542) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using CGRP antibody (CAB5542) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-CALCA Antibody (CAB13957)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)