The Calpain 1 Monoclonal Antibody (CAB8710) is a high-quality antibody developed for reliable detection and analysis of target proteins. Calpain-1 is a calcium-dependent protease that plays a crucial role in numerous cellular processes, including cell signaling, cytoskeletal remodeling, and apoptosis.This antibody, produced using rabbit monoclonal technology, has been validated for use in Western blotting, immunohistochemistry, and other laboratory techniques. It specifically binds to calpain-1, allowing for accurate detection and quantification in samples from different cell types and tissues.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Calpain 1 Monoclonal Antibody
SKU:
CAB8710
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1272
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
823
RRID
AB_2863593
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Calpain 1 Rabbit mAb (CAB8710) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunofluorescence analysis of C6 cells using Calpain 1 Rabbit mAb (CAB8710) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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