Anti-CAPN1 Antibody (CAB1172)
- SKU:
- CAB1172
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-CAPN1 Antibody |
| Antibody SKU: | CAB1172 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CAPN1 (NP_005177.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-549, BT-474, MCF7, Jurkat, PC-3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CAPN1 (NP_005177.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KQRA RELG LGRH ENAI KYLG QDYE QLRV RCLQ SGTL FRDE AFPP VPQS LGYK DLGP NSSK TYGI KWKR PTEL LSNP QFIV DGAT RTDI CQGA LGDC WLLA AIAS LTLN DTLL HRVV PHGQ SFQN GYAG IFHF QLWQ FGEW VDVV VDDL LPIK DGKL VFVH SAEG NEFW SALL EKAY AKVN GSYE ALSG GSTS EGFE DFTG GVTE WYEL RKAP SDLY QIIL KALE RGSL LGCS IDIS SVLD MEAI TFKK LVKG HAYS VTGA KQVN YRGQ VVSL IRMR NPWG EVEW TGAW SDSS SEWN NVDP YERD QLRV KME |
| Gene ID: | 823 |
| Uniprot: | P07384 |
| Cellular Location: | Cell membrane, Cytoplasm |
| Calculated MW: | 81kDa |
| Observed MW: | 82kDa |
| Synonyms: | CAPN1, CANP, CANP1, CANPL1, SPG76, muCANP, muCL, calpain 1 |
| Background: | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. |
| UniProt Protein Function: | CAPN1: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Ubiquitous. Activated by micromolar concentrations of calcium and inhibited by calpastatin. Belongs to the peptidase C2 family. |
| UniProt Protein Details: | Protein type:EC 3.4.22.52; Protease; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 11q13 Cellular Component: focal adhesion; membrane; cytoplasm; plasma membrane; cytosol Molecular Function:protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; receptor catabolic process; positive regulation of cell proliferation; proteolysis |
| NCBI Summary: | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
| UniProt Code: | P07384 |
| NCBI GenInfo Identifier: | 115574 |
| NCBI Gene ID: | 823 |
| NCBI Accession: | P07384.1 |
| UniProt Secondary Accession: | P07384,Q2TTR0, Q6DHV4, |
| UniProt Related Accession: | P07384 |
| Molecular Weight: | |
| NCBI Full Name: | Calpain-1 catalytic subunit |
| NCBI Synonym Full Names: | calpain 1, (mu/I) large subunit |
| NCBI Official Symbol: | CAPN1Â Â |
| NCBI Official Synonym Symbols: | CANP; muCL; CANP1; CANPL1; muCANPÂ Â |
| NCBI Protein Information: | calpain-1 catalytic subunit; CANP 1; calpain mu-type; micromolar-calpain; calpain-1 large subunit; calpain, large polypeptide L1; calcium-activated neutral proteinase 1; cell proliferation-inducing protein 30; cell proliferation-inducing gene 30 protein |
| UniProt Protein Name: | Calpain-1 catalytic subunit |
| UniProt Synonym Protein Names: | Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; Calpain-1 large subunit; Cell proliferation-inducing gene 30 protein; Micromolar-calpain; muCANP |
| Protein Family: | Calpain |
| UniProt Gene Name: | CAPN1Â Â |
| UniProt Entry Name: | CAN1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CAPN1 antibody (CAB1172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded mouse heart using CAPN1 antibody (CAB1172) at dilution of 1:100 (40x lens). |
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