Anti-Calretinin Antibody (CAB9807)

Antibody Name:	Anti-Calretinin Antibody
Antibody SKU:	CAB9807
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human CALB2 (NP_001731.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:1000 - 1:4000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, Mouse eye, Mouse brain, Rat spinal cord, Rat brain, Mouse small intestine, Mouse spinal cord, Rat eye

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human CALB2 (NP_001731.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGP QQQP PYLH LAEL TASQ FLEI WKHF DADG NGYI EGKE LENF FQEL EKAR KGSG MMSK SDNF GEKM KEFM QKYD KNSD GKIE MAEL AQIL PTEE NFLL CFRQ HVGS SAEF MEAW RKYD TDRS GYIE ANEL KGFL SDLL KKAN RPYD EPKL QEYT QTIL RMFD LNGD GKLG LSEM SRLL PVQE NFLL KFQG MKLT SEEF NAIF TFYD KDRS GYID EHEL DALL KDLY EKNK KEMN IQQL TNYR KSVM SLAE AGKL YRKD LEIV LCSE PPM
Gene ID:	794
Uniprot:	P22676
Cellular Location:
Calculated MW:	31kDa
Observed MW:	29kDa

Synonyms:

CALB2, CAB29, CAL2, CR

Background:

This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	CALB2: Calretinin is a calcium-binding protein which is abundant in auditory neurons. Belongs to the calbindin family.
UniProt Protein Details:	Protein type:Calcium-binding Chromosomal Location of Human Ortholog: 16q22.2 Cellular Component: cytoplasm; gap junction; nucleus Molecular Function:calcium ion binding Biological Process: cytosolic calcium ion homeostasis
NCBI Summary:	This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
UniProt Code:	P22676
NCBI GenInfo Identifier:	109940061
NCBI Gene ID:	794
NCBI Accession:	P22676.2
UniProt Secondary Accession:	P22676,Q53HD2, Q96BK4, A8K4Y1,
UniProt Related Accession:	P22676
Molecular Weight:	31,540 Da
NCBI Full Name:	Calretinin
NCBI Synonym Full Names:	calbindin 2
NCBI Official Symbol:	CALB2
NCBI Official Synonym Symbols:	CR; CAL2; CAB29
NCBI Protein Information:	calretinin; calbindin D29K; 29 kDa calbindin; calbindin 2, (29kD, calretinin)
UniProt Protein Name:	Calretinin
UniProt Synonym Protein Names:	29 kDa calbindin
UniProt Gene Name:	CALB2
UniProt Entry Name:	CALB2_HUMAN

	Western blot analysis of extracts of various cell lines, using CALB2 antibody (CAB9807) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded mouse kidney using CALB2 antibody (CAB9807) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using CALB2 antibody (CAB9807) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of rat brain using CALB2 antibody (CAB9807) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse brain using CALB2 antibody (CAB9807) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
CALB2 Antibody (PACO05360)	Human Calretinin ELISA Kit
CALB2 Antibody (PACO07244)	Human CR (Calretinin) CLIA Kit (HUES00415)
CALB2 Antibody (PACO08143)	Human CR (Calretinin) ELISA Kit (HUES01791)
	Human Calretinin (CALB2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)