The Camel LH (Luteinizing Hormone) ELISA Kit from Assay Genie is a cutting-edge assay designed for the quantitative detection of luteinizing hormone levels in camel serum, plasma, and tissue culture supernatants. With its exceptional sensitivity and specificity, this ELISA kit delivers accurate and consistent results, making it an invaluable tool for a diverse range of research applications. Luteinizing hormone is a key reproductive hormone in camels, playing a pivotal role in the regulation of the estrous cycle and ovulation. Monitoring LH levels is crucial for understanding camel reproductive physiology and optimizing breeding programs.
The Camel LH ELISA Kit enables researchers to explore camel reproduction in depth, shedding light on the mechanisms underlying fertility and aiding in the development of advanced reproductive technologies. With its user-friendly protocol and precise performance, the Camel LH ELISA Kit empowers researchers to unravel the complexities of camel reproductive biology and revolutionize the field of camel breeding. Trust Assay Genie's expertise to drive your camel research forward with reliable and insightful data.
Product Name:
Camel LH (Luteinizing Hormone) ELISA kit
SKU:
AEFI00030
Reactivity:
Camel
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
0.938 mIU/mL
Range:
1.563-100 mIU/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 10)
86-101%
84-95%
80-96%
EDTA Plasma (n = 10)
89-98%
86-99%
82-94%
Heparin Plasma (n = 10)
90-98%
85-101%
94-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 10)
96-104
99
EDTA Plasma (n = 10)
85-102
91
Heparin Plasma (n = 10)
86-104
96
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
