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Anti-CAMKK1 Antibody (CAB16716)

SKU:
CAB16716
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • ChIP Antibodies Anti-CAMKK1 Antibody CAB16716
  • ChIP Antibodies Anti-CAMKK1 Antibody CAB16716
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Description

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Antibody Name:Anti-CAMKK1 Antibody
Antibody SKU:CAB16716
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 399-520 of human CAMKK1 (NP_757344.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:LO2, HeLa, A-549
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 399-520 of human CAMKK1 (NP_757344.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LHRK IKNE PVVF PEEP EISE ELKD LILK MLDK NPET RIGV PDIK LHPW VTKN GEEP LPSE EEHC SVVE VTEE EVKN SVRL IPSW TTVI LVKS MLRK RSFG NPFE PQAR REER SMSA PGNL LV
Gene ID:84254
Uniprot:Q8N5S9
Cellular Location:Cytoplasm, Nucleus
Calculated MW:55kDa/57kDa
Observed MW:56kDa
Synonyms:CAMKK1, CAMKKA
Background:The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene.
UniProt Protein Function:CAMKK1: a calcium/calmodulin-dependent S/T protein kinase of the CAMKK family. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death. 2 isoforms of the human protein are produced by alternative splicing. The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition. Binding of calmodulin may releave intrasteric autoinhibition. May be regulated through phosphorylation by CAMK1 and CAMK4. Appears to be autophosphorylated in a Ca2+/calmodulin-dependent manner. Phosphorylation of Ser-458 is blocked upon binding to Ca2+/calmodulin.
UniProt Protein Details:

Protein type:Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.17; Other group; CAMKK family; Meta subfamily

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: cytosol

Molecular Function:calmodulin-dependent protein kinase activity; protein binding

NCBI Summary:The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q8N5S9
NCBI GenInfo Identifier:56404620
NCBI Gene ID:84254
NCBI Accession:Q8N5S9.2
UniProt Secondary Accession:Q8N5S9,Q9BQH3,
UniProt Related Accession:Q8N5S9
Molecular Weight:57,523 Da
NCBI Full Name:Calcium/calmodulin-dependent protein kinase kinase 1
NCBI Synonym Full Names:calcium/calmodulin dependent protein kinase kinase 1
NCBI Official Symbol:CAMKK1  
NCBI Official Synonym Symbols:CAMKKA  
NCBI Protein Information:calcium/calmodulin-dependent protein kinase kinase 1
UniProt Protein Name:Calcium/calmodulin-dependent protein kinase kinase 1
UniProt Synonym Protein Names:CaM-kinase IV kinase; Calcium/calmodulin-dependent protein kinase kinase alpha; CaM-KK alpha; CaM-kinase kinase alpha; CaMKK alpha
Protein Family:Calcium/calmodulin-dependent protein kinase
UniProt Gene Name:CAMKK1  
UniProt Entry Name:KKCC1_HUMAN
Anti-CAMKK1 Antibody (CAB16716)Western blot analysis of extracts of various cell lines, using CAMKK1 antibody (CAB16716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CAMKK1 Antibody (CAB16716)Immunohistochemistry of paraffin-embedded Human lung cancer using CAMKK1 Rabbit pAb (CAB16716) at dilution of 1:100 (40x lens).

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