The Canine MMP2 (Matrix Metalloproteinase 2) ELISA Kit is specifically designed for the quantitative detection of MMP2 levels in canine serum, plasma, and tissue homogenates. This kit offers high sensitivity and accuracy, ensuring precise and reliable results for research purposes. MMP2 is a key enzyme involved in the degradation of extracellular matrix proteins, playing a crucial role in tissue remodeling and wound healing. Abnormal MMP2 activity has been associated with various pathologies, including cancer, cardiovascular diseases, and inflammatory disorders.
Therefore, measuring MMP2 levels can provide valuable insights into disease progression and potential therapeutic interventions. With its user-friendly protocol and excellent performance, the Canine MMP2 ELISA Kit is an essential tool for studying MMP2 biology and its implications in canine health and disease. Trust Assay Genie for high-quality ELISA kits that deliver consistent and meaningful results for your research needs.
Serum, Plasma, Cell Culture Supernatant, Cell Lysis Buffer or Tissue Lysis Buffer, and Other Biological Fluid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
87-104%
95-105%
94-105%
EDTA Plasma (n = 5)
82-95%
86-101%
82-99%
Heparin Plasma (n = 5)
82-101%
82-94%
81-94%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-103
95
EDTA Plasma (n = 5)
87-105
96
Heparin Plasma (n = 5)
90-104
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
