Canine Monocyte Chemotactic Protein 2 (MCP2) ELISA Kit
The Canine Monocyte Chemotactic Protein 2 (MCP-2) ELISA Kit offered by Assay Genie is a cutting-edge tool for the precise measurement of MCP-2 levels in canine samples. This kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results for researchers working in the field of immunology and inflammation. MCP-2 is a key chemokine involved in the recruitment of monocytes, which play a crucial role in immune response and inflammatory processes.
By providing valuable insights into MCP-2 levels in various biological samples, this ELISA kit is ideal for studying canine inflammatory diseases, immune disorders, and potential therapeutic interventions. Trust Assay Genie to deliver high-quality research tools for your laboratory needs. Visit www.assaygenie.com to learn more.
Product Name:
Canine Monocyte Chemotactic Protein 2 (MCP2) ELISA Kit
Product Code:
CNDL00063
Size:
96 Assays
Target:
MCP2
Synonyms:
CCL8, CCL-8, SCYA8, MCP-2, HC14, Chemokine C-C-Motif Ligand 8, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 8, Monocyte Chemoattractant Protein 2
Detection Method:
Sandwich
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
