Canine N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit
The Canine N-terminal Pro-Brain Natriuretic Peptide (NT-proBNP) ELISA Kit from Assay Genie is specifically designed for the accurate detection of NT-proBNP levels in canine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications in veterinary medicine.NT-proBNP is a biomarker that plays a critical role in the assessment of cardiac function and the diagnosis of heart conditions in dogs. Elevated levels of NT-proBNP are associated with cardiac diseases such as congestive heart failure, making it a valuable tool for monitoring and managing cardiovascular health in canine patients.
By using the Canine NT-proBNP ELISA Kit, researchers and veterinarians can effectively measure NT-proBNP levels in dogs, providing valuable insights into their cardiac health and helping to guide treatment decisions. Trust Assay Genie for accurate and precise measurements that contribute to the well-being of our furry friends.
Product Name:
Canine N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit
Product Code:
CNDL00071
Size:
96 Assays
Target:
NT-ProBNP
Synonyms:
NT-Pro-BNP, N-BNP
Detection Method:
Competition
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
