Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
The Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit is designed for the quantitative detection of NT-proBNP levels in human serum, plasma, cell supernatant, lysates, and other biological samples. NT-proBNP is the inactive 76-amino acid N-terminal fragment cleaved from proBNP during the secretion of the active hormone BNP by cardiomyocytes in response to ventricular wall stress and volume overload. Due to its longer half-life and greater in vivo stability compared to BNP, NT-proBNP serves as a clinically important biomarker for the diagnosis and prognosis of heart failure, left ventricular dysfunction, and acute coronary syndromes. Elevated circulating levels of NT-proBNP are also observed in conditions such as hypertension, pulmonary embolism, renal dysfunction, and cardiotoxicity associated with chemotherapy. Accurate measurement of NT-proBNP is essential for understanding cardiovascular disease mechanisms and for developing targeted therapeutic strategies. Assay Genie's Human NT-proBNP ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and translational applications. Trust Assay Genie's Human NT-proBNP ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
SKU:
AEES05547
Size:
96T
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
~3 h 30 min
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Recovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NT-proBNP. Standards or samples are added to the wells, allowing the target to bind to the immobilized antibody. After incubation, a biotinylated detection antibody specific for Human NT-proBNP is added, which binds specifically to the captured target. Following a wash step to remove excess detection antibody, HRP-conjugated Streptavidin is introduced, forming a biotin-streptavidin-HRP complex. After a second washing step, Substrate Reagent is added to initiate a colorimetric reaction catalyzed by HRP. The reaction produces a blue product that turns yellow upon addition of the acidic Stop Solution. The optical density (OD) is measured at 450 nm using a microplate reader. The OD450 value is directly proportional to the concentration of NT-proBNP in the sample, which can be determined by referencing a standard curve.
