Anti-CAPRIN1 Antibody (CAB7910)

Antibody Name:	Anti-CAPRIN1 Antibody
Antibody SKU:	CAB7910
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human CAPRIN1

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, HeLa, Jurkat

Immunogen:	Recombinant protein of human CAPRIN1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4076
Uniprot:	Q14444
Cellular Location:	Cell projection, Cytoplasm, cytosol, dendrite
Calculated MW:	76kDa/78kDa
Observed MW:	120kDa

Synonyms:

CAPRIN1, GPIAP1, GPIP137, GRIP137, M11S1, RNG105, p137GPI, caprin-1

Background:

CAPRIN1(Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate, which suggests its essential for normal cellular proliferation.

UniProt Protein Function:	CAPRIN1: May regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Belongs to the caprin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; Cell surface Chromosomal Location of Human Ortholog: 11p13 Cellular Component: membrane; stress granule; integral to plasma membrane; cytoplasm; dendrite; cytosol Molecular Function:RNA binding Biological Process: negative regulation of translation; positive regulation of dendrite morphogenesis
UniProt Code:	Q14444
NCBI GenInfo Identifier:	182676426
NCBI Gene ID:	4076
NCBI Accession:	Q14444.2
UniProt Secondary Accession:	Q14444,Q15074, Q6IMN4, Q6IMN7, Q9BV09, A6NMY7, D3DR06
UniProt Related Accession:	Q14444
Molecular Weight:	709
NCBI Full Name:	Caprin-1
NCBI Synonym Full Names:	cell cycle associated protein 1
NCBI Official Symbol:	CAPRIN1
NCBI Official Synonym Symbols:	M11S1; GPIAP1; RNG105; GPIP137; GRIP137; p137GPI
NCBI Protein Information:	caprin-1; GPI-p137; caprin 1; RNA granule protein 105; GPI-anchored protein p137; GPI-anchored membrane protein 1; cell cycle-associated protein 1; activation/proliferation-associated protein 1; membrane component chromosome 11 surface marker 1; membrane
UniProt Protein Name:	Caprin-1
UniProt Synonym Protein Names:	Cell cycle-associated protein 1; Cytoplasmic activation- and proliferation-associated protein 1; GPI-anchored membrane protein 1; GPI-anchored protein p137; GPI-p137; p137GPI; Membrane component chromosome 11 surface marker 1; RNA granule protein 105
Protein Family:	Caprin
UniProt Gene Name:	CAPRIN1
UniProt Entry Name:	CAPR1_HUMAN

	Western blot analysis of extracts of various cell lines, using CAPRIN1 antibody (CAB7910) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using CAPRIN1 antibody (CAB7910) (40x lens).
	Immunofluorescence analysis of C6 cells using CAPRIN1 Rabbit pAb (CAB7910) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using CAPRIN1 Rabbit pAb (CAB7910) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using CAPRIN1 Rabbit pAb (CAB7910) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

CAPRIN1 Antibody (PACO08177)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)