Anti-CPB1 Antibody (CAB10486)

SKU:
CAB10486
€99 - €249

Description

Technical Manual

Anti-CPB1 Antibody

Product Name:Anti-CPB1 Antibody
Product SKU:CAB10486
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 111-417 of human CPB1 (NP_001862.2).
Sequence:

ATGHSYEKYNKWETIEAWTQQVATENPALISRSVIGTTFEGRAIYLLKVGKAGQNKPAIFMDCGFHAREWISPAFCQWFVREAVRTYGREIQVTELLDKLDFYVLPVLNIDGYIYTWTKSRFWRKTRSTHTGSSCIGTDPNRNFDAGWCEIGASRNPCDETYCGPAAESEKETKALADFIRNKLSSIKAYLTIHSYSQMMIYPYSYAYKLGENNAELNALAKATVKELASLHGTKYTYGPGATTIYPAAGGSDDWAYDQGIRYSFTFELRDTGRYGFLLPESQIRATCEETFLAIKYVASYVLEHLY

Gene ID:1360
Swiss Prot:P15086
Synonyms:CPB1; CPB; PASP; PCPB
Calculated MW:47kDa
Observed MW:47kDa
Reactivity:Human, Mouse, Rat
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:SW620, Mouse pancreas, Mouse liver, Mouse spleen, Rat pancreas
Cellular Location:Secreted

Product Images

Anti-CPB1 Antibody (CAB10486)

Western blot analysis of extracts of various cell lines, using CPB1 antibody (CAB10486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Uniprot Information

UniProt Protein Function:CPB1: Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq, Jul 2008]
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted; EC 3.4.17.2; Protease

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: extracellular region

Molecular Function:carboxypeptidase activity; zinc ion binding; metallocarboxypeptidase activity

Biological Process: proteolysis

NCBI Summary:Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq, Jul 2008]
UniProt Code:P15086
NCBI GenInfo Identifier:20532382
NCBI Gene ID:1360
NCBI Accession:P15086.4
UniProt Secondary Accession:P15086,O60834, Q53XJ0, Q96BQ8,
UniProt Related Accession:P15086
Molecular Weight:47,368 Da
NCBI Full Name:Carboxypeptidase B
NCBI Synonym Full Names:carboxypeptidase B1 (tissue)
NCBI Official Symbol:CPB1
NCBI Official Synonym Symbols:CPB; PASP; PCPB
NCBI Protein Information:carboxypeptidase B; pancreas-specific protein; pancreatic carboxypeptidase B; procarboxypeptidase B; protaminase; tissue carboxypeptidase B
UniProt Protein Name:Carboxypeptidase B
UniProt Synonym Protein Names:Pancreas-specific protein; PASP
UniProt Gene Name:CPB1
UniProt Entry Name:CBPB1_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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