The CARS Monoclonal Antibody (CAB0438) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for a wide range of applications in immunology and cancer research.Cars, also known as CoaA or cysteinyl-tRNA synthetase, plays a critical role in cellular metabolism by catalyzing the ligation of cysteine to its cognate tRNA molecule. This process is essential for protein synthesis and overall cell function. The Anti-Cars Antibody binds specifically to the Cars protein, allowing for accurate detection and analysis in various cell types and experimental settings.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
CARS Monoclonal Antibody
SKU:
CAB0438
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC2504
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CARS, MDBH, CYSRS, MCDDBH, MGC:11246
Positive Sample:
HeLa, A549, MCF7, U-87MG, Rat pancreas
Cellular Localization:
Cytoplasm.
Calculated MW:
85kDa
Observed MW:
85kDa
This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
833
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CARS Rabbit mAb (CAB0438) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using CARS Rabbit mAb (CAB0438) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
