Anti-CART Antibody (CAB5395)

Antibody Name:	Anti-CART Antibody
Antibody SKU:	CAB5395
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse,
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 28-116 of human CART (NP_004282.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse,
Positive Samples:	Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 28-116 of human CART (NP_004282.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QEDA ELQP RALD IYSA VDDA SHEK ELIE ALQE VLKK LKSK RVPI YEKK YGQV PMCD AGEQ CAVR KGAR IGKL CDCP RGTS CNSF LLKC L
Gene ID:	9607
Uniprot:	Q16568
Cellular Location:	Secreted
Calculated MW:	12kDa
Observed MW:	13kDa

Synonyms:

CART, CARTPT

Background:

This gene encodes a preproprotein that is proteolytically processed to generate multiple biologically active peptides. These peptides play a role in appetite, energy balance, maintenance of body weight, reward and addiction, and the stress response. Expression of a similar gene transcript in rodents is upregulated following administration of cocaine and amphetamine. Mutations in this gene are associated with susceptibility to obesity in humans.

UniProt Protein Function:	CARTPT: Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro. Belongs to the CART family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Secreted; Hormone; Secreted, signal peptide Chromosomal Location of Human Ortholog: 5q13.2 Cellular Component: extracellular space; secretory granule Molecular Function:protein binding Biological Process: negative regulation of osteoclast differentiation; activation of MAPKK activity; adult feeding behavior; cellular response to starvation; signal transduction; negative regulation of appetite; G-protein coupled receptor protein signaling pathway; synaptic transmission; cell-cell signaling; neuropeptide signaling pathway; positive regulation of epinephrine secretion; cell glucose homeostasis; circadian regulation of gene expression; negative regulation of bone resorption; regulation of insulin secretion; positive regulation of blood pressure; positive regulation of transmission of nerve impulse Disease: Obesity
NCBI Summary:	This gene encodes a secreted protein which is processed by prohormone/proprotein convertases to produce smaller, biologically active peptides. Expression of the transcript for this gene is regulated by certain drugs such as cocaine, and the encoded protein is thought to be involved in the regulation of appetite and stress. Mutations in this gene are associated with susceptibility to obesity.[provided by RefSeq, Nov 2009]
UniProt Code:	Q16568
NCBI GenInfo Identifier:	2833274
NCBI Gene ID:	9607
NCBI Accession:	Q16568.1
UniProt Secondary Accession:	Q16568,Q6FG92,
UniProt Related Accession:	Q16568
Molecular Weight:	12,829 Da
NCBI Full Name:	Cocaine- and amphetamine-regulated transcript protein
NCBI Synonym Full Names:	CART prepropeptide
NCBI Official Symbol:	CARTPT
NCBI Official Synonym Symbols:	CART
NCBI Protein Information:	cocaine- and amphetamine-regulated transcript protein; cocaine and amphetamine regulated transcript
UniProt Protein Name:	Cocaine- and amphetamine-regulated transcript protein
Protein Family:	Cocaine- and amphetamine-regulated transcript protein
UniProt Gene Name:	CARTPT
UniProt Entry Name:	CART_HUMAN

	Western blot analysis of extracts of mouse heart, using CART antibody (CAB5395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
	Immunofluorescence analysis of U2OS cells using CART Rabbit pAb (CAB5395) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-CARTPT Antibody (CAB18275)	Human CART / Cocaine- and amphetamine-regulated transcript ELISA Kit
CARTPT Antibody (PACO08190)	Human Cocaine- and amphetamine-regulated transcript protein (CARTPT) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)