Western blot analysis of extracts from rat lung, using Cathepsin E (CTSE) antibody (CAB22092) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Cathepsin E (CTSE) Monoclonal Antibody (CAB22092) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using the latest techniques, specifically targets the CTSE protein and is highly reactive with human samples.Cathepsin E is known for its role in MHC class II antigen presentation and has been implicated in various disease processes, including autoimmune disorders, infectious diseases, and cancer. The CTSE Monoclonal Antibody enables researchers to detect and analyze the expression of Cathepsin E in various cell types, providing valuable insights into its function and potential therapeutic applications.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cathepsin E (CTSE) Monoclonal Antibody
SKU:
CAB22092
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC55299
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CATE, Cathepsin E (CTSE)
Positive Sample:
NUGC-4, Rat thymus, Mouse stomach
Cellular Localization:
Endosome.
Calculated MW:
43kDa
Observed MW:
43kDa
This gene encodes a member of the A1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme, an aspartic endopeptidase, may be involved in antigen processing and the maturation of secretory proteins. Elevated expression of this gene has been observed in neurodegeneration.
Purification Method
Affinity purification
Gene ID
1510
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cathepsin E (CTSE) Rabbit mAb (CAB22092) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1 s.
Western blot analysis of lysates from Mouse stomach using Cathepsin E (CTSE) Rabbit mAb (CAB22092) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
