Human Cathepsin E/CTSE Recombinant Protein (RPES4653)

SKU:
RPES4653
Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
€196

Description

ELISA Kit Technical Manual

Recombinant Human Cathepsin E/CTSE Protein (His Tag)

Background:Cathepsin E (CTSE) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. It is a member of the Peptidase C1 family, and has a specificity similar to that of Pepsin A and Cathepsin D. CTSE is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome. It is expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. CTSE is an intracellular proteinase that have a role in immune function, activation-induced lymphocyte depletion in the thymus, neuronal degeneration and glial cell activation in the brain. Futhermore, it probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation.
Name:Recombinant Human Cathepsin E/CTSE Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES4653
Synonyms:Cathepsin E; CTSE
Species:Human
Expression host:Human Cells
Sequence:Ser20-Pro396
Accession:P14091
Mol Mass:41.8 kDa
AP Mol Mass:46 kDa
Tag:C-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM MES, 150mM NaCl, pH 5.5.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:CTSE: May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain. Belongs to the peptidase A1 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.4.23.34; Protease

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: endosome

Molecular Function:protein homodimerization activity; aspartic-type endopeptidase activity

Biological Process: protein autoprocessing; digestion; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis

NCBI Summary:The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen. Transcript variants utilizing alternative polyadenylation signals and two transcript variants encoding different isoforms exist for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P14091
NCBI GenInfo Identifier:46397366
NCBI Gene ID:1510
NCBI Accession:P14091.2
UniProt Secondary Accession:P14091,Q5TZ01, Q5TZ02, Q9NY58, Q9UCE3, Q9UCE4,
UniProt Related Accession:P14091
Molecular Weight:396
NCBI Full Name:Cathepsin E
NCBI Synonym Full Names:cathepsin E
NCBI Official Symbol:CTSE
NCBI Official Synonym Symbols:CATE
NCBI Protein Information:cathepsin E; slow-moving proteinase; erythrocyte membrane aspartic proteinase
UniProt Protein Name:Cathepsin E
Protein Family:Cathepsin
UniProt Gene Name:CTSE
UniProt Entry Name:CATE_HUMAN