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Anti-CBX8 Antibody (CAB6222)

SKU:
CAB6222
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-CBX8 Antibody CAB6222
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-CBX8 Antibody CAB6222
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-CBX8 Antibody CAB6222
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-CBX8 Antibody CAB6222
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-CBX8 Antibody CAB6222
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-CBX8 Antibody
Antibody SKU:CAB6222
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 80-280 of human CBX8 (NP_065700.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, 293T, HeLa, MCF-7, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 80-280 of human CBX8 (NP_065700.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LLKA QAKA KAKT YEFR SDSA RGIR IPYP GRSP QDLA STSR AREG LRNM GLSP PASS TSTS STCR AEAP RDRD RDRD RDRE RDRE RERE RERE RERE RERE RGTS RVDD KPSS PGDS SKKR GPKP RKEL PDPS QRPL GEPS AGLG EYLK GRKL DDTP SGAG KFPA GHSV IQLA RRQD SDLV QCGV TSPS SAEA TGKL AVDT F
Gene ID:57332
Uniprot:Q9HC52
Cellular Location:Nucleus
Calculated MW:43kDa
Observed MW:50kDa
Synonyms:CBX8, PC3, RC1
Background:
UniProt Protein Function:CBX8: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; heterochromatin; nuclear chromatin; PcG protein complex; nucleus

Molecular Function:protein binding; single-stranded RNA binding; ubiquitin-protein ligase activity; methylated histone residue binding

Biological Process: transcription, DNA-dependent; histone ubiquitination; negative regulation of transcription from RNA polymerase II promoter

UniProt Code:Q9HC52
NCBI GenInfo Identifier:78099843
NCBI Gene ID:57332
NCBI Accession:Q9HC52.3
UniProt Secondary Accession:Q9HC52,Q96H39, Q9NR07,
UniProt Related Accession:Q9HC52
Molecular Weight:389
NCBI Full Name:Chromobox protein homolog 8
NCBI Synonym Full Names:chromobox homolog 8
NCBI Official Symbol:CBX8  
NCBI Official Synonym Symbols:PC3; RC1  
NCBI Protein Information:chromobox protein homolog 8; polycomb 3; rectachrome 1; Pc class 3 homolog; chromobox homolog 8 (Pc class homolog, Drosophila)
UniProt Protein Name:Chromobox protein homolog 8
UniProt Synonym Protein Names:Polycomb 3 homolog; Pc3; hPc3; Rectachrome 1
Protein Family:Chromobox protein
UniProt Gene Name:CBX8  
UniProt Entry Name:CBX8_HUMAN
Anti-CBX8 Antibody (CAB6222)Western blot analysis of extracts of various cell lines, using CBX8 antibody (CAB6222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-CBX8 Antibody (CAB6222)Immunohistochemistry of paraffin-embedded rat pancreas using CBX8 Antibody (CAB6222) at dilution of 1:100 (40x lens).
Anti-CBX8 Antibody (CAB6222)Immunohistochemistry of paraffin-embedded human colon using CBX8 Antibody (CAB6222) at dilution of 1:100 (40x lens).
Anti-CBX8 Antibody (CAB6222)Immunohistochemistry of paraffin-embedded mouse testis using CBX8 Antibody (CAB6222) at dilution of 1:100 (40x lens).
Anti-CBX8 Antibody (CAB6222)Immunofluorescence analysis of U2OS cells using CBX8 antibody (CAB6222). Blue: DAPI for nuclear staining.

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