Anti-Cyclin A2 Antibody (CAB2891)

Antibody Name:	Anti-Cyclin A2 Antibody
Antibody SKU:	CAB2891
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Cyclin A2 (NP_001228.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HeLa, HL-60, MCF7, Mouse spleen, Rat thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Cyclin A2 (NP_001228.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLGN SAPG PATR EAGS ALLA LQQT ALQE DQEN INPE KAAP VQQP RTRA ALAV LKSG NPRG LAQQ QRPK TRRV APLK DLPV NDEH VTVP PWKA NSKQ PAFT IHVD EAEK EAQK KPAE SQKI ERED ALAF NSAI SLPG PRKP LVPL DYPM DGSF ESPH TMDM SIVL EDEK PVSV NEVP DYHE DIHT YLRE MEVK CKPK VGYM
Gene ID:	890
Uniprot:	P20248
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	48kDa
Observed MW:	55kDa

Synonyms:

CCN1, CCNA, Cyclin A2, CCNA2, cyclin-A2

Background:

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

UniProt Protein Function:	CCNA2: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
UniProt Protein Details:	Protein type:Cell cycle regulation; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 4q27 Cellular Component: nucleoplasm; male pronucleus; cytoplasm; female pronucleus; nucleus Molecular Function:protein binding; protein kinase binding Biological Process: mitosis; positive regulation of fibroblast proliferation; organ regeneration; response to glucagon stimulus; cell division; positive regulation of transcription, DNA-dependent; mitotic cell cycle G2/M transition DNA damage checkpoint; regulation of G2/M transition of mitotic cell cycle; Ras protein signal transduction; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; response to estradiol stimulus
NCBI Summary:	The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M. [provided by RefSeq, Aug 2016]
UniProt Code:	P20248
NCBI GenInfo Identifier:	311033358
NCBI Gene ID:	890
NCBI Accession:	P20248.2
UniProt Related Accession:	P20248
Molecular Weight:	~ 49kDa
NCBI Full Name:	Cyclin-A2
NCBI Synonym Full Names:	cyclin A2
NCBI Official Symbol:	CCNA2
NCBI Official Synonym Symbols:	CCN1; CCNA
NCBI Protein Information:	cyclin-A2
UniProt Protein Name:	Cyclin-A2
Protein Family:	Protein
UniProt Gene Name:	CCNA2
UniProt Entry Name:	CCNA2_HUMAN

	Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (CAB2891) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human prostate using Cyclin A2 antibody (CAB2891) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using Cyclin A2 antibody (CAB2891) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using Cyclin A2 antibody (CAB2891) at dilution of 1:100 (40x lens).

Antibodies

Anti-Cyclin A2 Antibody (CAB15036)

Anti-Cyclin A2 Antibody (CAB7632)

Anti-Cyclin A2 Antibody (CAB19036)

CCNA2 Antibody (PACO08243)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)