Cyclin A1 is a member of the highly conserved cyclin family that is characterized by a dramatic periodicity in protein abundance, and belongs to the A-type cyclin subfamily. The mammalian A-type cyclin family consists of two members: cyclin A1 and cyclin A2. Different cyclins exhibit distinct expression. Cyclin A1 is expressed in mice exclusively in the germ cell lineage and high rate of cyclinA1 is found in human testis and certain myeloid leukaemia cells. Cyclin A1 is primarily function in the control of meiosis. It serves as regulator subunits binding to cyclin-dependent kinase 1 (Cdk1) and cyclin-dependent kinase 2 (Cdk2), which give two different kinase activities, one appearing in S phase, the other in G2. Through this, cyclin A1 operate the entry and progression in cell cycle. High frequency of cyclin A1 overexpression has been observed in acute myelocytic leukemias, especially those that are at the promyelocyte and myeloblast stages of development.
Product Name:
Human CCNA1/Cyclin-A1 Recombinant Protein (RPES4344)
Product Code:
RPES4344
Size:
100 µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
CT146
Accession:
NP_003905.1
Sequence:
Met 1-Gln 465
Fusion tag:
N-His
Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 at 10 µg/ml (100 ?l/well) can bind biotinylated human CDK1. The EC50 of biotinylated human CDK1 is 0.02-0.04 µg/ml.Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 at 10 µg/ml (100 ?l/well) can bind biotinylated human CDK2-His. The EC50 of biotinylated human CDK2-His is 0.07-0.15 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the full length of human CCNA1 isoform a (NP_003905.1) (Met 1-Gln 465) was expressed, with a polyhistidine tag at the N-terminus.
Purity:
> 96 % as determined by reducing SDS-PAGE.
Mol Mass:
54.6 kDa
AP Mol Mass:
50 kDa
Formulation:
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
