Anti-Cyclin D1 Antibody (CAB11022)

Antibody Name:	Anti-Cyclin D1 Antibody
Antibody SKU:	CAB11022
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human Cyclin D1 (NP_444284.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HepG2, SW480, HT-1080, Mouse kidney

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human Cyclin D1 (NP_444284.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PSMV AAGS VVAA VQGL NLRS PNNF LSYY RLTR FLSR VIKC DPDC LRAC QEQI EALL ESSL RQAQ QNMD PKAA EEEE EEEE EVDL ACTP TDVR DVDI
Gene ID:	595
Uniprot:	P24385
Cellular Location:	Cytoplasm, Membrane, Nucleus
Calculated MW:	33kDa
Observed MW:	37kDa

Synonyms:

BCL1, D11S287E, PRAD1, U21B31, CCND1, Cyclin D1, cyclin D1

Background:

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

UniProt Protein Function:	CCND1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.
UniProt Protein Details:	Protein type:Activator; Cell cycle regulation; Nuclear receptor co-regulator; Oncoprotein Chromosomal Location of Human Ortholog: 11q13.3 Cellular Component: cyclin-dependent protein kinase holoenzyme complex; cytosol; intracellular; nucleoplasm; nucleus; transcriptional repressor complex Molecular Function:enzyme binding; histone deacetylase binding; protein binding; protein kinase binding; transcription corepressor activity; transcription factor binding Biological Process: G1 DNA damage checkpoint; G1/S transition of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell cycle; positive regulation of cyclin-dependent protein kinase activity; positive regulation of protein amino acid phosphorylation; response to DNA damage stimulus; response to drug Disease: Myeloma, Multiple; Von Hippel-lindau Syndrome
NCBI Summary:	The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]
UniProt Code:	P24385
NCBI GenInfo Identifier:	116152
NCBI Gene ID:	595
NCBI Accession:	P24385.1
UniProt Secondary Accession:	P24385,Q6LEF0,
UniProt Related Accession:	P24385
Molecular Weight:	34kDa
NCBI Full Name:	G1/S-specific cyclin-D1
NCBI Synonym Full Names:	cyclin D1
NCBI Official Symbol:	CCND1
NCBI Official Synonym Symbols:	BCL1; PRAD1; U21B31; D11S287E
NCBI Protein Information:	G1/S-specific cyclin-D1
UniProt Protein Name:	G1/S-specific cyclin-D1
UniProt Synonym Protein Names:	B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene
Protein Family:	G1/S-specific cyclin
UniProt Gene Name:	CCND1

	Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (CAB11022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon using Cyclin D1 Rabbit pAb (CAB11022) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using Cyclin D1 antibody (CAB11022) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug Cyclin D1 antibody (CAB11022). Western blot was performed from the immunoprecipitate using Cyclin D1 antibody (CAB11022) at a dilition of 1:1000.

Antibodies	ELISA Kits
Anti-Cyclin D1 Antibody (CAB0310)	Human Cyclin D1 ELISA Kit
Anti-Cyclin D1 Antibody (CAB11310)	Human CCND1 (Cyclin-D1) CLIA Kit (HUES01115)
Anti-Cyclin D1 Antibody (CAB2708)	Human G1/S-specific cyclin-D1 (CCND1) ELISA Kit
Anti-Phospho-CCND1-T286 Antibody (CABP0574)	Cyclin D1 (Phospho-Thr286) Colorimetric Cell-Based ELISA Kit
Anti-Cyclin D1 Antibody (CAB1301)	Cyclin D1 Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)