Anti-CCT4 Antibody (CAB6548)

Antibody Name:	Anti-CCT4 Antibody
Antibody SKU:	CAB6548
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 130-390 of human CCT4 (NP_006421.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, THP-1, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 130-390 of human CCT4 (NP_006421.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PTII SESF QKAL EKGI EILT DMSR PVEL SDRE TLLN SATT SLNS KVVS QYSS LLSP MSVN AVMK VIDP ATAT SVDL RDIK IVKK LGGT IDDC ELVE GLVL TQKV SNSG ITRV EKAK IGLI QFCL SAPK TDMD NQIV VSDY AQMD RVLR EERA YILN LVKQ IKKT GCNV LLIQ KSIL RDAL SDLA LHFL NKMK IMVI KDIE REDI EFIC KTIG TKPV AHID QFTA DMLG SAEL AEEV NLNG SGKL LKIT GCAS PGKT VTIV V
Gene ID:	10575
Uniprot:	P50991
Cellular Location:	Cytoplasm, Melanosome, centrosome, cilium basal body, cytoskeleton, microtubule organizing center
Calculated MW:	54kDa/57kDa
Observed MW:	60kDa

Synonyms:	CCT4, CCT-DELTA, Cctd, SRB
Background:

UniProt Protein Function:	CCT4: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Belongs to the TCP-1 chaperonin family.
UniProt Protein Details:	Protein type:Chaperone Chromosomal Location of Human Ortholog: 2p15 Cellular Component: centrosome; chaperonin-containing T-complex; cytoplasm; cytosol; microtubule; nucleoplasm Molecular Function:protein binding Biological Process: positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; protein folding; protein stabilization
NCBI Summary:	The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130), as well as alpha-transducin (MIM 139330) (Won et al., 1998 [PubMed 9819444]).[supplied by OMIM, Mar 2008]
UniProt Code:	P50991
NCBI GenInfo Identifier:	52001478
NCBI Gene ID:	10575
NCBI Accession:	P50991.4
UniProt Secondary Accession:	P50991,O14870, Q53QP9, Q96C51, B2R6I3, B7Z8B1, F5H5W3
UniProt Related Accession:	P50991
Molecular Weight:	54,720 Da
NCBI Full Name:	T-complex protein 1 subunit delta
NCBI Synonym Full Names:	chaperonin containing TCP1 subunit 4
NCBI Official Symbol:	CCT4
NCBI Official Synonym Symbols:	SRB; Cctd; CCT-DELTA
NCBI Protein Information:	T-complex protein 1 subunit delta
UniProt Protein Name:	T-complex protein 1 subunit delta
UniProt Synonym Protein Names:	CCT-delta; Stimulator of TAR RNA-binding
Protein Family:	T-complex protein
UniProt Gene Name:	CCT4
UniProt Entry Name:	TCPD_HUMAN

	Western blot analysis of extracts of various cell lines, using CCT4 antibody (CAB6548) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat stomach using CCT4 antibody (CAB6548) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using CCT4 antibody (CAB6548) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spinal cord using CCT4 antibody (CAB6548) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using CCT4 antibody (CAB6548) at dilution of 1:100 (40x lens).

Antibodies

CCT4 Antibody (PACO08262)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)