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Western blot analysis of Mouse spleen, using CD123 Rabbit pAb (CAB22111) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

CD123 Polyclonal Antibody (CAB22111)

Datasheet MSDS
SKU CAB22111
Product Type Antibody
Antibody Type Polyclonal Antibody
Reactivity Mouse
Host Species Rabbit
Synonyms IL3R, CD123, IL3RX, IL3RY, IL3RAY, hIL-3Ra, IL-3R-alpha
$127 - $488

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Description

system_update_altDatasheetsystem_update_altMSDS

CD123 Polyclonal Antibody (CAB22111)

The CD123 Polyclonal Antibody (CAB22111) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity and sensitivity for human samples, making it ideal for immunohistochemistry, flow cytometry, and other applications.CD123, also known as interleukin-3 receptor alpha chain, plays a crucial role in the development and function of hematopoietic cells, making it a key target for research in hematopoiesis, leukemia, and other blood disorders. The CD123 Polyclonal Antibody binds specifically to the CD123 protein, allowing for precise detection and analysis in various cell types and tissues.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.

Product Name:CD123 Polyclonal Antibody
SKU:CAB22111
Size:20μL, 100μL
Reactivity:Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:KEDP NPPI TNLR MKAK AQQL TWDL NRNV TDIE CVKD ADYS MPAV NNSY CQFG AISL CEVT NYTV RVAN PPFS TWIL FPEN SGKP WAGA ENLT CWIH DVDF LSCS WAVG PGAP ADVQ YDLY LNVA NRRQ QYEC LHYK TDAQ GTRI GCRF DDIS RLSS GSQS SHIL VRGR SAAF GIPC TDKF VVFS QIEI LTPP NMTA KCNK THSF MHWK MRSH FNRK FRYE LQIQ KRMQ PVIT EQVR DRTS FQLL NPGT YTVQ IRAR ERVY EFLS AWST PQRF ECDQ EEGA NTRA WR
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:IL3R, CD123, IL3RX, IL3RY, IL3RAY, hIL-3Ra, IL-3R-alpha
Positive Sample:Mouse spleen
Cellular Localization:External Side Of Plasma Membrane, Plasma Membrane.
Calculated MW:43kDa
Observed MW:70kDa

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found.

Purification MethodAffinity purification
Gene ID3563
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Mouse spleen, using CD123 Rabbit pAb (CAB22111) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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