The CD2 Antibody (CAB5632) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is optimized for use in Western blot applications. By binding specifically to the CD2 protein, it allows for the precise detection and analysis of this important immune cell marker in various cell types.CD2 is known to play a crucial role in T cell activation and adhesion, making it essential for studying immune responses and signaling pathways.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD2 Antibody
SKU:
CAB5632
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
T11, SRBC, LFA-2, CD2
Positive Sample:
Molt-4, Jurkat
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
39kDa
Observed MW:
39-58kDa
The protein encoded by this gene is a surface antigen found on all peripheral blood T-cells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3' flanking sequence of this gene.
Purification Method
Affinity purification
Gene ID
914
RRID
AB_2766392
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CD2 Rabbit pAb (CAB5632) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using CD2 Rabbit pAb (CAB5632) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
