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Anti-CD22 Antibody (CAB5790)

SKU:
CAB5790
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 9 Anti-CD22 Antibody CAB5790
  • Cell Biology Antibodies 9 Anti-CD22 Antibody CAB5790
€139 - €419
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Description

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Antibody Name:Anti-CD22 Antibody
Antibody SKU:CAB5790
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 708-847 of human CD22 (NP_001762.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:Human, Mouse
Positive Samples:Raji
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 708-847 of human CD22 (NP_001762.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LQRR WKRT QSQQ GLQE NSSG QSFF VRNK KVRR APLS EGPH SLGC YNPM MEDG ISYT TLRF PEMN IPRT GDAE SSEM QRPP PDCD DTVT YSAL HKRQ VGDY ENVI PDFP EDEG IHYS ELIQ FGVG ERPQ AQEN VDYV ILKH
Gene ID:933
Uniprot:P20273
Cellular Location:Cell membrane, Single-pass type I membrane protein
Calculated MW:75kDa/84kDa/85kDa/95kDa
Observed MW:135kDa
Synonyms:CD22, SIGLEC-2, SIGLEC2
Background:
UniProt Protein Function:CD22: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: integral to plasma membrane; external side of plasma membrane

Molecular Function:protein binding; carbohydrate binding

Biological Process: cell adhesion

UniProt Code:P20273
NCBI GenInfo Identifier:6166019
NCBI Gene ID:933
NCBI Accession:P20273.2
UniProt Secondary Accession:P20273,O95699, O95701, O95702, O95703, Q01665, Q32M46 Q92872, Q92873, Q9UQA6, F5GYU4, F5H7U3,
UniProt Related Accession:P20273
Molecular Weight:75,354 Da
NCBI Full Name:B-cell receptor CD22
NCBI Synonym Full Names:CD22 molecule
NCBI Official Symbol:CD22  
NCBI Official Synonym Symbols:SIGLEC2; SIGLEC-2  
NCBI Protein Information:B-cell receptor CD22; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2
UniProt Protein Name:B-cell receptor CD22
UniProt Synonym Protein Names:B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD_antigen: CD22
Protein Family:CD226 antigen
UniProt Gene Name:CD22  
UniProt Entry Name:CD22_HUMAN
Anti-CD22 Antibody (CAB5790)Western blot analysis of extracts of Raji cells, using CD22 Antibody (CAB5790) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021).
Anti-CD22 Antibody (CAB5790)Immunofluorescence analysis of U2OS cells using CD22 antibody (CAB5790). Blue: DAPI for nuclear staining.

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