The CD2AP Antibody (CAB7788) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, demonstrates high specificity and reactivity towards human samples, making it ideal for use in Western blot applications.CD2AP is known for its involvement in cell adhesion, T-cell activation, and immune receptor signaling pathways. By targeting the CD2AP protein, this antibody enables researchers to detect and analyze protein expression in various cell types, shedding light on its functions in immunology and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD2AP Antibody
SKU:
CAB7788
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CMS, CD2AP
Positive Sample:
A-431, Raji, MCF7, SW480, HL-60, Mouse kidney, Rat kidney
Cellular Localization:
Cell Projection, Cytoplasm, Cytoskeleton, Ruffle.
Calculated MW:
71kDa
Observed MW:
90kDa
This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.
Purification Method
Affinity purification
Gene ID
23607
RRID
AB_2768782
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CD2AP Rabbit pAb (CAB7788) at 1:500 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 60s.
