Anti-CD3E Antibody (CAB1753)

Antibody Name:	Anti-CD3E Antibody
Antibody SKU:	CAB1753
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-207 of human CD3E (NP_000724.1).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, Raji, MCF7, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-207 of human CD3E (NP_000724.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NEEM GGIT QTPY KVSI SGTT VILT CPQY PGSE ILWQ HNDK NIGG DEDD KNIG SDED HLSL KEFS ELEQ SGYY VCYP RGSK PEDA NFYL YLRA RVCE NCME MDVM SVAT IVIV DICI TGGL LLLV YYWS KNRK AKAK PVTR GAGA GGRQ RGQN KERP PPVP NPDY EPIR KGQR DLYS GLNQ RRI
Gene ID:	916
Uniprot:	P07766
Cellular Location:	Cell membrane, Single-pass type I membrane protein
Calculated MW:	23kDa
Observed MW:	23kDa

Synonyms:

CD3E, IMD18, T3E, TCRE, CD3e molecule

Background:

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.

UniProt Protein Function:	CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.
UniProt Protein Details:	Protein type:Receptor, misc.; Membrane protein, integral Chromosomal Location of Human Ortholog: 11q23 Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane Molecular Function:protein binding; transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; receptor signaling protein activity; T cell receptor binding; SH3 domain binding; protein kinase binding Biological Process: regulation of immune response; T cell activation; positive regulation of interleukin-2 biosynthetic process; signal complex assembly; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; positive regulation of interleukin-4 production; regulation of apoptosis; G-protein coupled receptor protein signaling pathway; positive regulation of interferon-gamma production; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; protein complex assembly; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation; positive regulation of T cell anergy; transmembrane receptor protein tyrosine kinase signaling pathway; response to nutrient Disease: Immunodeficiency 18
NCBI Summary:	The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
UniProt Code:	P07766
NCBI GenInfo Identifier:	1345708
NCBI Gene ID:	916
NCBI Accession:	P07766.2
UniProt Related Accession:	P07766
Molecular Weight:
NCBI Full Name:	T-cell surface glycoprotein CD3 epsilon chain
NCBI Synonym Full Names:	CD3e molecule
NCBI Official Symbol:	CD3E
NCBI Official Synonym Symbols:	T3E; TCRE; IMD18
NCBI Protein Information:	T-cell surface glycoprotein CD3 epsilon chain
UniProt Protein Name:	T-cell surface glycoprotein CD3 epsilon chain
UniProt Synonym Protein Names:	T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e
UniProt Gene Name:	CD3E
UniProt Entry Name:	CD3E_HUMAN

	Western blot analysis of extracts of various cell lines, using CD3E Antibody (CAB1753) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human tonsil using CD3E Antigen antibody (CAB1753) at dilution of 1:200 (40x lens).

Antibodies	ELISA Kits
Human CD3 Monoclonal Antibody [OKT3](AGEL0055)	Human CD3e / CD3 epsilon ELISA Kit
Human CD3 Monoclonal Antibody (Biotin Conjugated) [OKT3](AGEL0056)	Human T-cell surface glycoprotein CD3 epsilon chain (CD3E) ELISA Kit
Human CD3 Monoclonal Antibody (FITC Conjugated) [OKT3](AGEL0057)
Human CD3 Monoclonal Antibody (PE Conjugated) [OKT3](AGEL0058)
Human CD3 Monoclonal Antibody (APC Conjugated) [OKT3](AGEL0059)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)