The CD80 Antibody (CAB16039) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. RRID AB_2763477 Gene ID 941 Swiss Prot Synonym B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1; CD80
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD80 Antibody
SKU:
CAB16039
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISAIF-P
Recommended Dilution:
WB
1:500 - 1:1000
IF-P
1:50 - 1:200
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1, CD80
Positive Sample:
Rat lung
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
33kDa
Observed MW:
50-75kDa
The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. RRID AB_2763477 Gene ID 941 Swiss Prot Synonym B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1; CD80
Purification Method:
Affinity purification
Gene ID:
941
RRID:
AB_2763477
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat lung, using CD80 Rabbit pAb (CAB16039) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
WWestern blot analysis of various lysates, using CD80 Rabbit pAb (CAB16039) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunofluorescence analysis of Mouse spleen tissue using CD80 Rabbit pAb (CAB16039) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.
