The CD83 Monoclonal Antibody (CAB4234) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD83 Monoclonal Antibody
SKU:
CAB4234
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0935
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:2000
IF-P
1:100 - 1:400
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BL11, HB15, CD83
Positive Sample:
HeLa, Raji, U-937, Mouse liver, Mouse brain, Rat brain
Cellular Localization:
Membrane, .
Calculated MW:
23kDa
Observed MW:
23-65kDa
The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
9308
RRID
AB_2863215
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CD83 Rabbit mAb (CAB4234) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunofluorescence analysis of paraffin-embedded rat spleen using CD83 Rabbit mAb (CAB4234) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
