Anti-CD84 Antibody (CAB6433)

Antibody Name:	Anti-CD84 Antibody
Antibody SKU:	CAB6433
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-225 of human CD84 (NP_003865.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human
Positive Samples:	Jurkat, THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-225 of human CD84 (NP_003865.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KDSE IFTV NGIL GESV TFPV NIQE PRQV KIIA WTSK TSVA YVTP GDSE TAPV VTVT HRNY YERI HALG PNYN LVIS DLRM EDAG DYKA DINT QADP YTTT KRYN LQIY RRLG KPKI TQSL MASV NSTC NVTL TCSV EKEE KNVT YNWS PLGE EGNV LQIF QTPE DQEL TYTC TAQN PVSN NSDS ISAR QLCA DIAM GFRT HHTG
Gene ID:	8832
Uniprot:	Q9UIB8
Cellular Location:	Cell membrane, Single-pass type I membrane protein
Calculated MW:	24kDa/26kDa/30kDa/31kDa/36kDa/38kDa
Observed MW:	65kDa

Synonyms:

CD84, LY9B, SLAMF5, hCD84, mCD84

Background:

This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	CD84: Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain- containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Immunoglobulin superfamily; Motility/polarity/chemotaxis; Cell surface Chromosomal Location of Human Ortholog: 1q24 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:protein binding Biological Process: adaptive immune response; blood coagulation; defense response; homophilic cell adhesion; innate immune response; leukocyte migration; negative regulation of granulocyte macrophage colony-stimulating factor production; negative regulation of interleukin-18 production; negative regulation of mast cell activation; negative regulation of mast cell degranulation
NCBI Summary:	This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]
UniProt Code:	Q9UIB8
NCBI GenInfo Identifier:	74762772
NCBI Gene ID:	8832
NCBI Accession:	Q9UIB8.1
UniProt Secondary Accession:	Q9UIB8,O15430, O95266, O95660, Q5H9R1, Q6FHA8, Q8WLP1 Q8WWI8, Q9UF04, Q9UIB6, B2R8T1, B7Z3R8,
UniProt Related Accession:	Q9UIB8
Molecular Weight:	24,025 Da
NCBI Full Name:	SLAM family member 5
NCBI Synonym Full Names:	CD84 molecule
NCBI Official Symbol:	CD84
NCBI Official Synonym Symbols:	LY9B; hCD84; mCD84; SLAMF5
NCBI Protein Information:	SLAM family member 5
UniProt Protein Name:	SLAM family member 5
UniProt Synonym Protein Names:	Cell surface antigen MAX.3; Hly9-beta; Leukocyte differentiation antigen CD84; Signaling lymphocytic activation molecule 5; CD_antigen: CD84
Protein Family:	SLAM family
UniProt Gene Name:	CD84
UniProt Entry Name:	SLAF5_HUMAN

	Western blot analysis of extracts of various cell lines, using CD84 antibody (CAB6433) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of HeLa cells using CD84 antibody (CAB6433). Blue: DAPI for nuclear staining.

Antibodies

CD84 Antibody (PACO01961)

CD84 Antibody (PACO08302)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)